Access the full text.
Sign up today, get DeepDyve free for 14 days.
G. Canonica, I. Ansotegui, R. Pawankar, P. Schmid‐Grendelmeier, M. Hage, C. Baena-Cagnani, G. Melioli, C. Nunes, G. Passalacqua, L. Rosenwasser, H. Sampson, J. Sastre, J. Bousquet, T. Zuberbier (2013)
A WAO - ARIA - GA²LEN consensus document on molecular-based allergy diagnosticsThe World Allergy Organization Journal, 6
Robert Hamilton, P. Williams (2010)
Human IgE antibody serology: a primer for the practicing North American allergist/immunologist.The Journal of allergy and clinical immunology, 126 1
R. Hamilton (2010)
Clinical laboratory assessment of immediate-type hypersensitivity.The Journal of allergy and clinical immunology, 125 2 Suppl 2
M. Chapman, F. Ferreira, M. Villalba, O. Cromwell, D. Bryan, W. Becker, M. Fernández-Rivas, S. Durham, S. Vieths, R. Ree (2008)
The European Union CREATE project: a model for international standardization of allergy diagnostics and vaccines.The Journal of allergy and clinical immunology, 122 5
A. Asarnoj, C. Nilsson, J. Lidholm, S. Glaumann, E. Östblom, G. Hedlin, M. Hage, G. Lilja, M. Wickman (2012)
Peanut component Ara h 8 sensitization and tolerance to peanut.The Journal of allergy and clinical immunology, 130 2
Sven Pokoj, I. Lauer, K. Fötisch, M. Himly, A. Mari, E. Enrique, M. Miguel-Moncín, J. Lidholm, S. Vieths, S. Scheurer (2010)
Pichia pastoris is superior to E. coli for the production of recombinant allergenic non-specific lipid-transfer proteins.Protein expression and purification, 69 1
F. Fenaille, E. Nony, H. Chabre, Aurélie Lautrette, M. Couret, T. Batard, P. Moingeon, E. Ezan (2009)
Mass spectrometric investigation of molecular variability of grass pollen group 1 allergens.Journal of proteome research, 8 8
R. Hiller, S. Laffer, C. Harwanegg, M. Huber, W. Schmidt, A. Twardosz, B. Barletta, W. Becker, K. Blaser, H. Breiteneder, M. Chapman, R. Crameri, M. Duchêne, F. Ferreira, H. Fiebig, K. Hoffmann‐Sommergruber, T. King, T. Kleber-Janke, V. Kurup, S. Lehrer, J. Lidholm, U. Müller, C. Pini, G. Reese, O. Scheiner, A. Scheynius, Horng‐Der Shen, S. Spitzauer, R. Suck, I. Swoboda, W. Thomas, R. Tinghino, M. Hage-Hamsten, T. Virtanen, D. Kraft, Manfred Müller, R. Valenta (2002)
Microarrayed allergen molecules: diagnostic gatekeepers for allergy treatmentThe FASEB Journal, 16
N. Mungroo, Suresh Neethirajan (2014)
Biosensors for the Detection of Antibiotics in Poultry Industry—A ReviewBiosensors, 4
M. Chapman, P. Briza (2012)
Molecular Approaches to Allergen StandardizationCurrent Allergy and Asthma Reports, 12
C. Incorvaia, M. Mauro, E. Ridolo, E. Makrì, M. Montagni, G. Ciprandi (2015)
A Pitfall to Avoid When Using an Allergen Microarray: The Incidental Detection of IgE to Unexpected Allergens.The journal of allergy and clinical immunology. In practice, 3 6
Ola Nilsson, T. Neimert-Andersson, Mattias Bronge, J. Grundström, Ranjana Sarma, H. Uchtenhagen, A. Kikhney, T. Sandalova, Erik Holmgren, D. Svergun, A. Achour, M. Hage, H. Grönlund (2014)
Designing a Multimer Allergen for Diagnosis and Immunotherapy of Dog Allergic PatientsPLoS ONE, 9
U. Seppälä, C. Dauly, Sarah Robinson, M. Hornshaw, J. Larsen, H. Ipsen (2011)
Absolute quantification of allergens from complex mixtures: a new sensitive tool for standardization of allergen extracts for specific immunotherapy.Journal of proteome research, 10 4
B. Zybailov, A. Mosley, Mihaela Sardiu, Michael Coleman, L. Florens, M. Washburn (2006)
Statistical analysis of membrane proteome expression changes in Saccharomyces cerevisiae.Journal of proteome research, 5 9
G. Feyzkhanova, G. Feyzkhanova, M. Filippova, V. Talibov, E. Dementieva, V. Maslennikov, Yu.P. Reznikov, N. Offermann, Alexander Zasedatelev, Alexander Zasedatelev, A. Rubina, M. Fooke-Achterrath (2014)
Development of hydrogel biochip for in vitro allergy diagnostics.Journal of immunological methods, 406
E. King, L. Vailes, A. Tsay, S. Satinover, M. Chapman (2007)
Simultaneous detection of total and allergen-specific IgE by using purified allergens in a fluorescent multiplex array.The Journal of allergy and clinical immunology, 120 5
G. Gadermaier, M. Hauser, F. Ferreira (2014)
Allergens of weed pollen: an overview on recombinant and natural molecules.Methods, 66 1
M. Chapman, A. Pomés, H. Breiteneder, F. Ferreira (2007)
Nomenclature and structural biology of allergens.The Journal of allergy and clinical immunology, 119 2
Geoff Platt, F. Damin, M. Swann, I. Metton, G. Skorski, M. Cretich, M. Chiari (2014)
Allergen immobilisation and signal amplification by quantum dots for use in a biosensor assay of IgE in serum.Biosensors & bioelectronics, 52
C. Lupinek, E. Wollmann, A. Baar, Srinita Banerjee, H. Breiteneder, Barbara Broecker, M. Bublin, M. Curin, S. Flicker, T. Garmatiuk, H. Hochwallner, I. Mittermann, S. Pahr, Y. Resch, K. Roux, Bharani Srinivasan, S. Stentzel, S. Vrtala, Leanna Willison, M. Wickman, K. Lodrup-Carlsen, J. Anto, J. Bousquet, C. Bachert, D. Ebner, T. Schlederer, C. Harwanegg, R. Valenta (2014)
Advances in allergen-microarray technology for diagnosis and monitoring of allergy: the MeDALL allergen-chip.Methods, 66 1
Amit Joshi, M. Peczuh, C. Kumar, J. Rusling (2014)
Ultrasensitive carbohydrate-peptide SPR imaging microarray for diagnosing IgE mediated peanut allergy.The Analyst, 139 22
S. Thorpe, A. Heath, B. Fox, D. Patel, W. Egner (2014)
The 3rd International Standard for serum IgE: international collaborative study to evaluate a candidate preparationClinical Chemistry and Laboratory Medicine (CCLM), 52
S. Vieths, D. Barber, M. Chapman, A. Costanzo, A. Daas, H. Fiebig, K. Hanschmann, M. Hrabina, S. Kaul, A. Ledesma, P. Moingeon, G. Reese, C. Schörner, R. Ree, B. Weber, K. Buchheit (2012)
Establishment of recombinant major allergens Bet v 1 and Phl p 5a as Ph. Eur. reference standards and validation of ELISA methods for their measurement. Results from feasibility studies.Pharmeuropa bio & scientific notes, 2012
Stefan Wöhrl, K. Vigl, S. Zehetmayer, R. Hiller, R. Jarisch, M. Prinz, G. Stingl, T. Kopp (2006)
The performance of a component‐based allergen‐microarray in clinical practiceAllergy, 61
D. Pomponi, M. Bernardi, M. Liso, P. Palazzo, L. Tuppo, Chiara Rafaiani, Mario Santoro, Alexis Labrada, M. Ciardiello, A. Mari, E. Scala (2012)
Allergen Micro-Bead Array for IgE Detection: A Feasibility Study Using Allergenic Molecules Tested on a Flexible Multiplex Flow Cytometric ImmunoassayPLoS ONE, 7
R. Ree, M. Chapman, F. Ferreira, S. Vieths, D. Bryan, O. Cromwell, M. Villalba, S. Durham, W. Becker, M. Aalbers, C. André, D. Barber, A. Bahima, A. Custovic, A. Didierlaurent, C. Dolman, J. Dorpema, G. Felice, F. Eberhardt, E. Caldas, M. Rivas, H. Fiebig, M. Focke, K. Fötisch, G. Gadermaier, R. Das, E. Mancebo, M. Himly, T. Kinaciyan, A. Knulst, A. Kroon, U. Lepp, F. Marco, A. Mari, P. Moingeon, R. Monsalve, A. Neubauer, S. Notten, P. Heer, G. Pauli, C. Pini, A. Purohit, J. Quiralte, S. Rak, M. Raulf‐Heimsoth, M. Moncín, B. Simpson, A. Tsay, L. Vailes, M. Wallner, B. Weber (2008)
EU Forum: The CREATE Project: development of certified reference materials for allergenic products and validation of methods for their quantificationAllergy, 63
E. Erwin, N. Custis, S. Satinover, M. Perzanowski, J. Woodfolk, J. Crane, K. Wickens, T. Platts-Mills (2005)
Quantitative measurement of IgE antibodies to purified allergens using streptavidin linked to a high-capacity solid phase.The Journal of allergy and clinical immunology, 115 5
W. Thomas (2011)
The advent of recombinant allergens and allergen cloning.The Journal of allergy and clinical immunology, 127 4
Kirsten Beyer, Kirsten Beyer, Linus Grabenhenrich, M. Härtl, A. Beder, B. Kalb, M. Ziegert, A. Finger, N. Harandi, R. Schlags, M. Gappa, L. Puzzo, H. Röblitz, M. Millner-Uhlemann, S. Büsing, H. Ott, Lars Lange, Bodo Niggemann, Bodo Niggemann (2015)
Predictive values of component‐specific IgE for the outcome of peanut and hazelnut food challenges in childrenAllergy, 70
M. Chapman (2010)
Lateral Flow Tests for Allergy Diagnosis: Point-of-Care or Point of Contention?International Archives of Allergy and Immunology, 152
Allergy diagnostics is being transformed by the advent of in vitro IgE testing using purified allergen molecules, combined with multiplex technology and biosensors, to deliver discriminating, sensitive, and high-throughput molecular diagnostics at the point of care. Essential elements of IgE molecular diagnostics are purified natural or recombinant allergens with defined purity and IgE reactivity, planar or bead-based multiplex systems to enable IgE to multiple allergens to be measured simultaneously, and, most recently, nanotechnology-based biosensors that facilitate rapid reaction rates and delivery of test results via mobile devices. Molecular diagnostics relies on measurement of IgE to purified allergens, the “active ingredients” of allergenic extracts. Typically, this involves measuring IgE to multiple allergens which is facilitated by multiplex technology and biosensors. The technology differentiates between clinically significant cross-reactive allergens (which could not be deduced by conventional IgE assays using allergenic extracts) and provides better diagnostic outcomes. Purified allergens are manufactured under good laboratory practice and validated using protein chemistry, mass spectrometry, and IgE antibody binding. Recently, multiple allergens (from dog) were expressed as a single molecule with high diagnostic efficacy. Challenges faced by molecular allergy diagnostic companies include generation of large panels of purified allergens with known diagnostic efficacy, access to flexible and robust array or sensor technology, and, importantly, access to well-defined serum panels form allergic patients for product development and validation. Innovations in IgE molecular diagnostics are rapidly being brought to market and will strengthen allergy testing at the point of care.
Current Allergy and Asthma Reports – Springer Journals
Published: Jul 5, 2015
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.