Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Synthesis of L-lactate oxidaze in yeast Yarrowia lipolytica during submerged cultivation

Synthesis of L-lactate oxidaze in yeast Yarrowia lipolytica during submerged cultivation The biosynthesis of L-lactate oxidase in the Yarrowia lipolytica yeast during submerged cultivation in laboratory bioreactors ANKUM-2M has been studied. It has been shown under optimal conditions of yeast cultivation with L-lactate that 24.5 U/L enzyme accumulated in the medium and the yield was 2.0 U/(L h). An increase in the biosynthesis of L-lactate oxidase to 75 U/L and the yield to 3.2 U/(L h) was achieved in the medium with L-lactate (1%) and glucose (2%). The enzyme was purified 251 times to homogeneity by hydrophobic and ion exchange chromatography state with a yield of 45% and a specific activity of 55.3 U/mg. Techniques of gel filtration and denaturing electrophoresis showed that L-lactate oxidase from Y. lipolytica is a tetramer with a molecular mass of 200–230 kDa. The enzyme showed a strict specificity to L-lactate and did not oxidize fumarate, pyruvate, succinate, ascorbate, dihydroxyacetone, glycolate, D-lactate, D, L-2-hydroxybutyrate and D, L-alanine or D-serine. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Applied Biochemistry and Microbiology Springer Journals

Synthesis of L-lactate oxidaze in yeast Yarrowia lipolytica during submerged cultivation

Download PDF
5 pages

Loading next page...
 
/lp/springer-journals/synthesis-of-l-lactate-oxidaze-in-yeast-yarrowia-lipolytica-during-nw87mUoOdI

References (9)

  • E.N. Biryukova (2009)

    650

    Microbiology (Moscow), 78

  • H. Sztajer (1996)

    600

    Appl. Microbiol. Biotechnol., 45

  • J.D. Duncan (1989)

    919

    Biochem. Biophys. Res. Commun., 164

  • M.B. Kupletskaya (2007)

    178

    Appl. Biochem. Microbiol., 43

  • S. Takemori (1968)

    313

    J. Biol. Chem., 243

  • N. Tzanetakis (1989)

    859

    J. Dairy Sci., 72

  • T. Atsusi (1998)

    507

    J. Ferment. Bioeng., 85

  • S.A. Streitenberger (2002)

    657

    Biotechnol. Prog., 18

  • P.A. Sullivan (1977)

    375

    Biochem. J., 165

Publisher
Springer Journals
Copyright
Copyright © 2017 by Pleiades Publishing, Inc.
Subject
Life Sciences; Biochemistry, general; Microbiology; Medical Microbiology
ISSN
0003-6838
eISSN
1608-3024
DOI
10.1134/S0003683817020065
Publisher site
See Article on Publisher Site

Abstract

The biosynthesis of L-lactate oxidase in the Yarrowia lipolytica yeast during submerged cultivation in laboratory bioreactors ANKUM-2M has been studied. It has been shown under optimal conditions of yeast cultivation with L-lactate that 24.5 U/L enzyme accumulated in the medium and the yield was 2.0 U/(L h). An increase in the biosynthesis of L-lactate oxidase to 75 U/L and the yield to 3.2 U/(L h) was achieved in the medium with L-lactate (1%) and glucose (2%). The enzyme was purified 251 times to homogeneity by hydrophobic and ion exchange chromatography state with a yield of 45% and a specific activity of 55.3 U/mg. Techniques of gel filtration and denaturing electrophoresis showed that L-lactate oxidase from Y. lipolytica is a tetramer with a molecular mass of 200–230 kDa. The enzyme showed a strict specificity to L-lactate and did not oxidize fumarate, pyruvate, succinate, ascorbate, dihydroxyacetone, glycolate, D-lactate, D, L-2-hydroxybutyrate and D, L-alanine or D-serine.

Journal

Applied Biochemistry and Microbiology – Springer Journals

Published: Mar 10, 2017

There are no references for this article.