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(1972)
Metody biokhimicheskogo issledovaniya rastenii (Meth ods of Biochemical Plant Research)
- Publisher
- Springer Journals
- Copyright
- Copyright © 2010 by Pleiades Publishing, Ltd.
- Subject
- Life Sciences; Microbiology; Medical Microbiology; Biochemistry, general
- ISSN
- 0003-6838
- eISSN
- 1608-3024
- DOI
- 10.1134/S0003683811050152
- Publisher site
- See Article on Publisher Site
Abstract
Synthesis of 3- and 4-hydroxybutyrate copolymer (3HB-co-4HB), the most promising member of the biodegradable polyhydroxyalkanoate (PHA) family, has been studied. Cultivation conditions of naturally occurring strains of hydrogen-oxidizing bacteria Ralstonia eutropha B5786 and Cupriavidus eutrophus B10646 have been optimized to ensure efficient synthesis of the 3HB-co-4HB copolymer. A set of highly pure samples of the 3HB-co-4HB copolymer with 4HB content varying from 8.7 to 24.3 mol% has been obtained. Incorporation of 4HB into the copolymer was shown to cause a more pronounced decrease in polymer crystallinity than the incorporation of 3-hydroxyvalerate or 3-hydroxyhexanoate; samples with a degree of crystallinity below 30% have been obtained. The weight average molecular mass of the 3HB-co-4HB copolymers was shown to be independent on the monomer ratio and to vary broadly (from 540 to 1110 kDa).
Journal
Applied Biochemistry and Microbiology – Springer Journals
Published: Sep 7, 2011