Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Study of the Effect of Lower Eukaryotes on Tacrolimus (FK-506) Biosynthesis by the Streptomyces tsukubensis Strain VKM Ac-2618D

Study of the Effect of Lower Eukaryotes on Tacrolimus (FK-506) Biosynthesis by the Streptomyces... The effect of inactivated yeast cells, individual components of the yeast cells, and the inactivated biomass of filamentous fungi (micromycetes) of various taxonomic positions on the biosynthesis of immunosuppressant tacrolimus (FK-506) by Streptomyces tsukubensis VKM Ac-2618D cells has been studied. The fractions of soluble and insoluble components of yeast cells positively influenced tacrolimus biosynthesis; the FK-506 concentrations reached 447.1± 22.4 mg/L and 514.3 ± 25.7 mg/L, respectively. The insoluble cell components, including the cell walls and cytoplasmic membranes, had a greater effect than other fractions. The presence of whole baker’s yeast cells and the filamentous fungus Aspergillus ochraceus biomass in the complex nutrient medium stimulated tacrolimus production by S. tsukubensis VKM Ac-2618D (667.5 ± 33.4 mg/L and 633.5 ± 31.7 mg/L, respectively). The biomass of deuteromycete Curvularia lunata had no effect on FK‑506 biosynthesis (288.4 ± 14.4 mg/L). Conversely, the biomass of the Gongronella butleri fungus fully inhibited growth and tacrolimus production in S. tsukubensis. The increase in FK-506 biosynthesis in the presence of the cells of the tested micromycetes is more likely due to the polysaccharide composition of their cell walls than to the ergosterol content. The results expand the knowledge of S. tsukubensis physiology and may be useful for the development of the biotechnological process of tacrolimus production. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Applied Biochemistry and Microbiology Springer Journals

Study of the Effect of Lower Eukaryotes on Tacrolimus (FK-506) Biosynthesis by the Streptomyces tsukubensis Strain VKM Ac-2618D

Download PDF
7 pages

Loading next page...
 
/lp/springer-journals/study-of-the-effect-of-lower-eukaryotes-on-tacrolimus-fk-506-lxj0802Iks

References (47)

Publisher
Springer Journals
Copyright
Copyright © Pleiades Publishing, Inc. 2020. ISSN 0003-6838, Applied Biochemistry and Microbiology, 2020, Vol. 56, No. 8, pp. 847–853. © Pleiades Publishing, Inc., 2020. Russian Text © The Author(s), 2019, published in Biotekhnologiya, 2019, Vol. 35, No. 5, pp. 42–50.
ISSN
0003-6838
eISSN
1608-3024
DOI
10.1134/S0003683820080062
Publisher site
See Article on Publisher Site

Abstract

The effect of inactivated yeast cells, individual components of the yeast cells, and the inactivated biomass of filamentous fungi (micromycetes) of various taxonomic positions on the biosynthesis of immunosuppressant tacrolimus (FK-506) by Streptomyces tsukubensis VKM Ac-2618D cells has been studied. The fractions of soluble and insoluble components of yeast cells positively influenced tacrolimus biosynthesis; the FK-506 concentrations reached 447.1± 22.4 mg/L and 514.3 ± 25.7 mg/L, respectively. The insoluble cell components, including the cell walls and cytoplasmic membranes, had a greater effect than other fractions. The presence of whole baker’s yeast cells and the filamentous fungus Aspergillus ochraceus biomass in the complex nutrient medium stimulated tacrolimus production by S. tsukubensis VKM Ac-2618D (667.5 ± 33.4 mg/L and 633.5 ± 31.7 mg/L, respectively). The biomass of deuteromycete Curvularia lunata had no effect on FK‑506 biosynthesis (288.4 ± 14.4 mg/L). Conversely, the biomass of the Gongronella butleri fungus fully inhibited growth and tacrolimus production in S. tsukubensis. The increase in FK-506 biosynthesis in the presence of the cells of the tested micromycetes is more likely due to the polysaccharide composition of their cell walls than to the ergosterol content. The results expand the knowledge of S. tsukubensis physiology and may be useful for the development of the biotechnological process of tacrolimus production.

Journal

Applied Biochemistry and Microbiology – Springer Journals

Published: Dec 1, 2020

There are no references for this article.