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Streptomyces variabilis Isolate MW091521: a New Microbial Source of Heliomycin

Streptomyces variabilis Isolate MW091521: a New Microbial Source of Heliomycin A total of 192 different Streptomyces isolates were recovered from 34 soil samples collected from different localities in Egypt. The cytotoxic activities of culture supernatant of each of the recovered isolate were investigated against Caco-2 cell line using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The Streptomyces isolate coded S19, that showed the highest cytotoxic activity against Caco-2 cell line (CD50 of 26.4 µg/mL), was identified using 16S rRNA gene sequence analysis as Streptomyces variabilis isolate MW091521. The influence of cultural parameters affecting the cytotoxic activity of the respective isolate including the different culture media, initial pH value, temperature and rotation speed was investigated. A maximum cytotoxic activity (CD50 of 13.4 µg/mL) was obtained using the culture medium of soybean meal at an initial pH of 7.0, incubation temperature 28°C and 200 rpm. Thereafter, the fermentation was conducted in a 14 L laboratory fermenter under the previously stated optimum conditions with the aim of extraction, and purification of cytotoxic metabolite(s). Fractionation of the ethyl acetate extract followed by advanced spectroscopic analysis using LC/ESI/MS as well as 1D and 2D NMR experiments yielded the cytotoxic metabolite heliomycin. To the best of our knowledge, this is the first report on the production of heliomycin by Streptomyces variabilis isolate MW091521. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Applied Biochemistry and Microbiology Springer Journals

Streptomyces variabilis Isolate MW091521: a New Microbial Source of Heliomycin

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Publisher
Springer Journals
Copyright
Copyright © Pleiades Publishing, Inc. 2021. ISSN 0003-6838, Applied Biochemistry and Microbiology, 2021, Vol. 57, No. 5, pp. 564–570. © Pleiades Publishing, Inc., 2021.
ISSN
0003-6838
eISSN
1608-3024
DOI
10.1134/s0003683821050021
Publisher site
See Article on Publisher Site

Abstract

A total of 192 different Streptomyces isolates were recovered from 34 soil samples collected from different localities in Egypt. The cytotoxic activities of culture supernatant of each of the recovered isolate were investigated against Caco-2 cell line using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The Streptomyces isolate coded S19, that showed the highest cytotoxic activity against Caco-2 cell line (CD50 of 26.4 µg/mL), was identified using 16S rRNA gene sequence analysis as Streptomyces variabilis isolate MW091521. The influence of cultural parameters affecting the cytotoxic activity of the respective isolate including the different culture media, initial pH value, temperature and rotation speed was investigated. A maximum cytotoxic activity (CD50 of 13.4 µg/mL) was obtained using the culture medium of soybean meal at an initial pH of 7.0, incubation temperature 28°C and 200 rpm. Thereafter, the fermentation was conducted in a 14 L laboratory fermenter under the previously stated optimum conditions with the aim of extraction, and purification of cytotoxic metabolite(s). Fractionation of the ethyl acetate extract followed by advanced spectroscopic analysis using LC/ESI/MS as well as 1D and 2D NMR experiments yielded the cytotoxic metabolite heliomycin. To the best of our knowledge, this is the first report on the production of heliomycin by Streptomyces variabilis isolate MW091521.

Journal

Applied Biochemistry and MicrobiologySpringer Journals

Published: Sep 1, 2021

Keywords: Streptomyces variabilis; heliomycin; cytotoxic activity; Bioassay guided fractionation

References