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ISSN 0096-3925, Moscow University Biological Sciences Bulletin, 2019, Vol. 74, No. 2, pp. 75–80. © Allerton Press, Inc., 2019. Russian Text © The Author(s), 2019, published in Vestnik Moskovskogo Universiteta, Seriya 16: Biologiya, 2019, Vol. 74, No. 2, pp. 94–100. RESEARCH ARTICLE Some Peculiarities in Application of Denaturating and Non-Denaturating In Situ Hybridization on Chromosomes of Cereals a,b a,c a,b b V. M. Kuznetsova , O. V. Razumova , G. I. Karlov , T. X. Dang , a,b a,b, P. Yu. Kroupin , and M. G. Divashuk * Laboratory of Applied Genomics and Crop Breeding, All-Russia Research Institute of Agricultural Biotechnology, Moscow, 127550 Russia Center for Molecular Biotechnology, Russian State Agrarian University (MTAA), Moscow, 127550 Russia Laboratory of Molecular Systematics, Tsitsin Main Botanical Garden, Russian Academy of Sciences, Moscow, 127276 Russia *e-mail: divashuk@gmail.com Received December 17, 2018; revised April 17, 2019; accepted April 23, 2019 Abstract—Non-denaturing f luorescent in situ hybridization (ND FISH) is a convenient method of cytoge- netic research. Compared to the standard method, ND FISH is fast and easy to perform and requires less time, reagents, and tools. Thus, it is gaining increasing popularity among different groups of scientists and is used to accomplish various scientific
Moscow University Biological Sciences Bulletin – Springer Journals
Published: Sep 13, 2019
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