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Selection of the Optimal Enzyme Composition for Sugar Beet Pulp Conversion

Selection of the Optimal Enzyme Composition for Sugar Beet Pulp Conversion ISSN 0003-6838, Applied Biochemistry and Microbiology, 2019, Vol. 55, No. 6, pp. 677–683. © Pleiades Publishing, Inc., 2019. Russian Text © The Author(s), 2019, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2019, Vol. 55, No. 6, pp. 586–593. Selection of the Optimal Enzyme Composition for Sugar Beet Pulp Conversion a, a a a b M. V. Semenova *, A. M. Rozhkova , D. O. Osipov , A. D. Satrutdinov , O. A. Sinitsyna , a a a, b E. A. Rubtsova , E. G. Kondrateva , and A. P. Sinitsyn Fundamentals of Biotechnology Federal Research Center, Russian Academy of Sciences, 119071 Moscow, Russia Lomonosov Moscow State University, Chemistry Department, 119991 Moscow, Russia *e-mail: margs@mail.ru Received December 27, 2018; revised April 8, 2019; accepted June 20, 2019 Abstract—It was shown that the presence of cellobiohydrolase, β-glucosidase, endoglucanase, arabinoxylan- arabinofuranhydrolase, pectin lyase, and polygalacturonase is necessary for efficient hydrolysis of sugar beet pulp (SBP). Optimal multienzyme complexes consisting of the same enzymes and additional endoarabinase or exoarabinase, endogalactanase, β-xylosidase, endoxylanase, and/or α-arabinofuranosidase were deter- mined. These components enabled the conversion of SBP based on a yield of total reducing sugars (RSs) of 61– 68%, an arabinose yield of 94%, and a glucose http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Applied Biochemistry and Microbiology Springer Journals

Selection of the Optimal Enzyme Composition for Sugar Beet Pulp Conversion

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References (1)

Publisher
Springer Journals
Copyright
Copyright © 2019 by Pleiades Publishing, Inc.
Subject
Life Sciences; Biochemistry, general; Microbiology; Medical Microbiology
ISSN
0003-6838
eISSN
1608-3024
DOI
10.1134/S0003683819050119
Publisher site
See Article on Publisher Site

Abstract

ISSN 0003-6838, Applied Biochemistry and Microbiology, 2019, Vol. 55, No. 6, pp. 677–683. © Pleiades Publishing, Inc., 2019. Russian Text © The Author(s), 2019, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2019, Vol. 55, No. 6, pp. 586–593. Selection of the Optimal Enzyme Composition for Sugar Beet Pulp Conversion a, a a a b M. V. Semenova *, A. M. Rozhkova , D. O. Osipov , A. D. Satrutdinov , O. A. Sinitsyna , a a a, b E. A. Rubtsova , E. G. Kondrateva , and A. P. Sinitsyn Fundamentals of Biotechnology Federal Research Center, Russian Academy of Sciences, 119071 Moscow, Russia Lomonosov Moscow State University, Chemistry Department, 119991 Moscow, Russia *e-mail: margs@mail.ru Received December 27, 2018; revised April 8, 2019; accepted June 20, 2019 Abstract—It was shown that the presence of cellobiohydrolase, β-glucosidase, endoglucanase, arabinoxylan- arabinofuranhydrolase, pectin lyase, and polygalacturonase is necessary for efficient hydrolysis of sugar beet pulp (SBP). Optimal multienzyme complexes consisting of the same enzymes and additional endoarabinase or exoarabinase, endogalactanase, β-xylosidase, endoxylanase, and/or α-arabinofuranosidase were deter- mined. These components enabled the conversion of SBP based on a yield of total reducing sugars (RSs) of 61– 68%, an arabinose yield of 94%, and a glucose

Journal

Applied Biochemistry and MicrobiologySpringer Journals

Published: Dec 6, 2019

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