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A. Collmer, J.L. Reid, M.S. Mount (1988)
Methods in Enzymology
ISSN 0003-6838, Applied Biochemistry and Microbiology, 2019, Vol. 55, No. 6, pp. 677–683. © Pleiades Publishing, Inc., 2019. Russian Text © The Author(s), 2019, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2019, Vol. 55, No. 6, pp. 586–593. Selection of the Optimal Enzyme Composition for Sugar Beet Pulp Conversion a, a a a b M. V. Semenova *, A. M. Rozhkova , D. O. Osipov , A. D. Satrutdinov , O. A. Sinitsyna , a a a, b E. A. Rubtsova , E. G. Kondrateva , and A. P. Sinitsyn Fundamentals of Biotechnology Federal Research Center, Russian Academy of Sciences, 119071 Moscow, Russia Lomonosov Moscow State University, Chemistry Department, 119991 Moscow, Russia *e-mail: margs@mail.ru Received December 27, 2018; revised April 8, 2019; accepted June 20, 2019 Abstract—It was shown that the presence of cellobiohydrolase, β-glucosidase, endoglucanase, arabinoxylan- arabinofuranhydrolase, pectin lyase, and polygalacturonase is necessary for efficient hydrolysis of sugar beet pulp (SBP). Optimal multienzyme complexes consisting of the same enzymes and additional endoarabinase or exoarabinase, endogalactanase, β-xylosidase, endoxylanase, and/or α-arabinofuranosidase were deter- mined. These components enabled the conversion of SBP based on a yield of total reducing sugars (RSs) of 61– 68%, an arabinose yield of 94%, and a glucose
Applied Biochemistry and Microbiology – Springer Journals
Published: Dec 6, 2019
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