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Screening of Upstream Transcript Factors of IbMYB1-1 by Yeast One-hybrid in Purple-fleshed Sweet Potato

Screening of Upstream Transcript Factors of IbMYB1-1 by Yeast One-hybrid in Purple-fleshed Sweet... Transcription factors IbMYB1-1 had been identified involving anthocyanins biosynthesis in purple-flesh sweet potato. However, the upstream transcription factors on PIbMYB1-1 involed in anthocyanin biosynthesis had rarely been reported. In this study, the upstream transcription factors on PIbMYB1-1 were screened by yeast one-hybrid screening in purple-fleshed sweet potato storage roots. The results indicated that IbJOX4, IbWRKY1, IbSCF and IbEIN3-2 had been identified as the upstream transcription factors of PIbMYB1-1 to regulated anthocyanin biosynthesis in purple-fleshed sweet potato. The interaction between the promoters and upstream transcription factors were verified by yeast one-hybrid and luciferase reporter assays. Subcellular localization analysis indicated that IbWRKY1, IbSCF and IbEIN3-2 were nuclear proteins and functioned as transcription factors to regulate expression of downstream genes. IbWRKY1, IbSCF and IbEIN3-2 had been identified as the upstream transcription factors of PIbMYB1-1 involved in anthocyanin biosynthesis in purple-fleshed sweet potato. The results provided a possible underlying mechanism of anthocyanin biosynthesis and a potential understanding of the regulatory network in purple-fleshed sweet potato. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Tropical Plant Biology Springer Journals

Screening of Upstream Transcript Factors of IbMYB1-1 by Yeast One-hybrid in Purple-fleshed Sweet Potato

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Publisher
Springer Journals
Copyright
Copyright © The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2022
ISSN
1935-9756
eISSN
1935-9764
DOI
10.1007/s12042-022-09309-7
Publisher site
See Article on Publisher Site

Abstract

Transcription factors IbMYB1-1 had been identified involving anthocyanins biosynthesis in purple-flesh sweet potato. However, the upstream transcription factors on PIbMYB1-1 involed in anthocyanin biosynthesis had rarely been reported. In this study, the upstream transcription factors on PIbMYB1-1 were screened by yeast one-hybrid screening in purple-fleshed sweet potato storage roots. The results indicated that IbJOX4, IbWRKY1, IbSCF and IbEIN3-2 had been identified as the upstream transcription factors of PIbMYB1-1 to regulated anthocyanin biosynthesis in purple-fleshed sweet potato. The interaction between the promoters and upstream transcription factors were verified by yeast one-hybrid and luciferase reporter assays. Subcellular localization analysis indicated that IbWRKY1, IbSCF and IbEIN3-2 were nuclear proteins and functioned as transcription factors to regulate expression of downstream genes. IbWRKY1, IbSCF and IbEIN3-2 had been identified as the upstream transcription factors of PIbMYB1-1 involved in anthocyanin biosynthesis in purple-fleshed sweet potato. The results provided a possible underlying mechanism of anthocyanin biosynthesis and a potential understanding of the regulatory network in purple-fleshed sweet potato.

Journal

Tropical Plant BiologySpringer Journals

Published: Jun 1, 2022

Keywords: IbJOX4; IbWRKY1; IbSCF; IbEIN3-2; Upstream transcription factors; Yeast one-hybrid screening

References