Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Screening of ornamental plants from the Botanic Gardens of Melbourne and Adelaide for the occurrence of Erwinia amylovora

Screening of ornamental plants from the Botanic Gardens of Melbourne and Adelaide for the... Host plants of Erwinia amylovora in the Royal Botanic Gardens of Melbourne (RBGM) and the Adelaide Botanic Gardens (ABG) were inspected for symptoms of fire blight including dieback, and shoot and stem cankers. Forty five symptomatic plants were sampled and concurrently tested for the presence of E. amylovora at the Institute for Horticultural Development, Victoria; Macquarie University, New South Wales; and the Max Planck Institute, Germany. At Max Planck, E. amylovora was isolated from wood samples from a Cotoneaster (Ag0002) and a Sorbus sorbus (Ag0034) from RBGM, and from a soft agar culture of bacteria isolated in Australia from Ag0002 and forwarded to Germany. Wood samples from ABG tested negative. Identification was confirmed by tests on selective media, by polymerase chain reaction assays, and by reactions on tobacco, pear slices and juvenile apple plants. The patterns obtained by pulsed field gel electrophoresis of the strains isolated from Australian samples in Germany and New Zealand were identical with each other and with strains of E. amylovora from Central Europe, New Zealand and with the English standard strain Ea595. However, the German and New Zealand strains of E. amylovora isolated from Australian wood samples differed in the size of the short DNA sequence repeat within the amplified plasmid pEA29 fragment. Exhaustive attempts in both Australian laboratories to isolate E. amylovora by similar test protocols from wood samples from RBGM and ABG were unsuccessful. Extensive surveys since the original outbreak have not detected the disease or the pathogen. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Australasian Plant Pathology Springer Journals

Screening of ornamental plants from the Botanic Gardens of Melbourne and Adelaide for the occurrence of Erwinia amylovora

Download PDF
9 pages

Loading next page...
 
/lp/springer-journals/screening-of-ornamental-plants-from-the-botanic-gardens-of-melbourne-5Ni0SV7iIL
Publisher
Springer Journals
Copyright
Copyright © 2000 by Australasian Plant Pathology Society
Subject
Life Sciences; Plant Pathology; Plant Sciences; Agriculture; Entomology; Ecology
ISSN
0815-3191
eISSN
1448-6032
DOI
10.1071/AP00020
Publisher site
See Article on Publisher Site

Abstract

Host plants of Erwinia amylovora in the Royal Botanic Gardens of Melbourne (RBGM) and the Adelaide Botanic Gardens (ABG) were inspected for symptoms of fire blight including dieback, and shoot and stem cankers. Forty five symptomatic plants were sampled and concurrently tested for the presence of E. amylovora at the Institute for Horticultural Development, Victoria; Macquarie University, New South Wales; and the Max Planck Institute, Germany. At Max Planck, E. amylovora was isolated from wood samples from a Cotoneaster (Ag0002) and a Sorbus sorbus (Ag0034) from RBGM, and from a soft agar culture of bacteria isolated in Australia from Ag0002 and forwarded to Germany. Wood samples from ABG tested negative. Identification was confirmed by tests on selective media, by polymerase chain reaction assays, and by reactions on tobacco, pear slices and juvenile apple plants. The patterns obtained by pulsed field gel electrophoresis of the strains isolated from Australian samples in Germany and New Zealand were identical with each other and with strains of E. amylovora from Central Europe, New Zealand and with the English standard strain Ea595. However, the German and New Zealand strains of E. amylovora isolated from Australian wood samples differed in the size of the short DNA sequence repeat within the amplified plasmid pEA29 fragment. Exhaustive attempts in both Australian laboratories to isolate E. amylovora by similar test protocols from wood samples from RBGM and ABG were unsuccessful. Extensive surveys since the original outbreak have not detected the disease or the pathogen.

Journal

Australasian Plant Pathology – Springer Journals

Published: Jan 28, 2011

References

  • Identification of Erwinia amylovora by PCR with chromosomal DNA
    Bereswill, S.; Bugert, P.; Bruchmuller, I.; Geider, K.
  • Molecular characterisation of natural Erwinia amylovora strains deficient in levan synthesis
    Bereswill, S.; Jock, S.; Aldridge, P.; Janse, J.D.; Geider, K.
  • Identification of Erwinia amylovora by growth in the presence of copper sulfate and by capsule staining with lectin
    Bereswill, S.; Jock, S.; Bellemann, P.; Geider, K.
  • Sensitive and species-specific detection of Erwinia amylovora by PCR-analysis
    Bereswill, S.; Pahl, A.; Bellemann, P.; Zeller, W.; Geider, K.
  • The Orchard; The outbreak of fire blight
    Campbell, J.A.
  • A selective medium for and a definitive colony characteristic of Erwinia amylovora
    Crosse, J.E.; Goodman, R.N.
  • A taxonomic study of the genus Erwinia. I. The Amylovora group
    Dye, D.W.
  • PCR-based techniques for the detection of Erwinia amylovora
    Guilford, P. J.; Taylor, R. K.; Clark, R.G.; Hale, C.N.; Forster, R.L.
  • The taxonomic significance of fermentative versus oxidative metabolism of carbohydrates by various Gram-negative bacteria
    Hugh, R.; Leifson, E.
  • A new medium for detecting Erwinia amylovora and its use in epidemiological studies
    Ishimaru, C.; Klos, E.J.
  • Isolation and characterization of Erwinia amylovora from Australian wood samples. International Workshop on Fire Blight 1998
    Jock, S.; Kim, W.-S.; Geider, K.; Rodoni, B; Merriman, P.
  • Fire blight in Australia? Proceedings of the International Symposium in Aschersleben, Germany 1998. “New aspects of Resistance Research on Cultivated Plants, Bacterial Diseases”
    Jock, S.; Kim, W.-S.; Rodoni, B.; Merriman, P.; Geider, K.
  • Analysis of variable short-sequence DNA repeats on the 29 kb plasmid of Erwinia amylovora strains
    Kim, W.-S.; Geider, K.
  • Two simple media for the demonstration of pyocyanin and fluorescin
    King, E.O.; Ward, M.K.; Raney, D.E.
  • The hypersensitive reaction to infection by bacterial plant pathogens
    Klement, Z.; Goodman, R. N.
  • Identification by PCR analysis on plasmid pEA29 of isolates of Erwinia amylovora responsible for an outbreak in Central Europe
    Leconte, P.; Manceau, C.; Paulin, J.-P.; Keck, M.
  • Detection of Erwinia amylovora by nested PCR and PCR-Dot-Blot and Reverse-Blot hybridisations
    McManus, P.S.; Jones, A.L.
  • Monitoring the epiphytic population of Erwinia amylovora on pear with a selective medium
    Miller, T.D.; Schroth, M.N.
  • Fire blight: Epidemiology and control (1921–1996)
    Paulin, J.-P.
  • Detection of Erwinia amylovora, the causal agent of fire blight, in the Royal Botanic Gardens, Melbourne, Australia. International Workshop on Fire Blight 1998
    Rodoni, B.; Kinsella, M.; Gardner, R.; Merriman, P.; Gillings, M.; Geider, K.
  • Instability of apEA29 marker in Erwinia amylovora previously used for strain classification
    Schnabel, E.; Jones, A.
  • Identification and characterisation of isolates of Erwinia amylovora from cotoneaster in Australia
    Taylor, R.; Hale, C.
  • Present worldwide distribution of fire blight
    Zwet, T.
  • Presence of Erwinia amylovora in apparently healthy nursery propagation material
    Zwet, T.; Walter, J.
  • Differentiation of Erwinia amylovora strains by pulsed-field gel electrophoresis
    Zhang, Y.; Geider, K.
  • Genomic analysis by pulsed-field gel electrophoresis of Erwinia amylovora strains from the Mediterranean region including Italy
    Zhang, Y.; Merighi, M.; Bazzi, C.; Geider, K.