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Salt-induced protein precipitation in aqueous solution: Single and binary protein systems

Salt-induced protein precipitation in aqueous solution: Single and binary protein systems Abstract A molecular-thermodynamic model is developed for the salt-induced protein precipitation. The protein molecules interact through four intermolecular potentials. An equation of state is derived based on the statistical mechanical perturbation theory with the modifiedChiew’s equation for the fluid phase, Young’s equation for the solid phase as the reference system and a perturbation based on the protein-protein effective two body potential. The equation of state provides an expression for the chemical potential of the protein. In a single protein system, the phase separation is represented by fluid-fluid equilibria. The precipitation behaviors are simulated with the partition coefficient at various salt concentrations and degree of pre-aggregation effect for the protein particles. In a binary protein system, we regard the system as a fluid-solid phase equilibrium. At equilibrium, we compute the reduced osmotic pressure-composition diagram in the diverse protein size difference and salt concentrations. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png "Macromolecular Research" Springer Journals

Salt-induced protein precipitation in aqueous solution: Single and binary protein systems

Kim, Sang Gon; Bae, Young Chan
"Macromolecular Research" , Volume 11 (1): 9 – Jan 1, 2003
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References (32)

Publisher
Springer Journals
Copyright
2003 The Polymer Society of Korea and Springer
ISSN
1598-5032
eISSN
2092-7673
DOI
10.1007/BF03218278
Publisher site
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Abstract

Abstract A molecular-thermodynamic model is developed for the salt-induced protein precipitation. The protein molecules interact through four intermolecular potentials. An equation of state is derived based on the statistical mechanical perturbation theory with the modifiedChiew’s equation for the fluid phase, Young’s equation for the solid phase as the reference system and a perturbation based on the protein-protein effective two body potential. The equation of state provides an expression for the chemical potential of the protein. In a single protein system, the phase separation is represented by fluid-fluid equilibria. The precipitation behaviors are simulated with the partition coefficient at various salt concentrations and degree of pre-aggregation effect for the protein particles. In a binary protein system, we regard the system as a fluid-solid phase equilibrium. At equilibrium, we compute the reduced osmotic pressure-composition diagram in the diverse protein size difference and salt concentrations.

Journal

"Macromolecular Research" – Springer Journals

Published: Jan 1, 2003

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