Access the full text.
Sign up today, get DeepDyve free for 14 days.
C. Wetzel, S. Rodermel (1998)
Regulation of phytoene desaturase expression is independent of leaf pigment content in Arabidopsis thalianaPlant Molecular Biology, 37
J. Watson, A. Fusaro, Ming-Bo Wang, P. Waterhouse (2005)
RNA silencing platforms in plantsFEBS Letters, 579
N. Jannoo, L. Grivet, Marc Seguin, F. Paulet, R. Domaingue, P. Rao, A. Dookun, A. D'Hont, J. Glaszmann (1999)
Molecular investigation of the genetic base of sugarcane cultivarsTheoretical and Applied Genetics, 99
J. Vickers, C. Grof, G. Bonnett, P. Jackson, D. Knight, S. Roberts, S. Robinson (2005)
Overexpression of polyphenol oxidase in transgenic sugarcane results in darker juice and raw sugarCrop Science, 45
J. Groenewald, F. Botha (2008)
Down-regulation of pyrophosphate: fructose 6-phosphate 1-phosphotransferase (PFP) activity in sugarcane enhances sucrose accumulation in immature internodesTransgenic Research, 17
R. Bower, A. Elliott, B. Potier, R. Birch (2004)
High-efficiency, microprojectile-mediated cotransformation of sugarcane, using visible or selectable markersMolecular Breeding, 2
A. Fusaro, L. Matthew, N. Smith, Shaun Curtin, Jasmina Dedic‐Hagan, Geoff Ellacott, J. Watson, Ming-Bo Wang, C. Brosnan, B. Carroll, P. Waterhouse (2006)
RNA interference‐inducing hairpin RNAs in plants act through the viral defence pathwayEMBO reports, 7
J. Sambrook, E. Fritsch, T. Maniatis (2001)
Molecular Cloning: A Laboratory Manual
R. Casu, C. Dimmock, S. Chapman, C. Grof, C. McIntyre, G. Bonnett, J. Manners (2004)
Identification of Differentially Expressed Transcripts from Maturing Stem of Sugarcane by in silico Analysis of Stem Expressed Sequence Tags and Gene Expression ProfilingPlant Molecular Biology, 54
Harry Smith (2000)
Phytochromes and light signal perception by plants—an emerging synthesisNature, 407
Stephen Mudge, K. Osabe, R. Casu, G. Bonnett, J. Manners, R. Birch (2009)
Efficient silencing of reporter transgenes coupled to known functional promoters in sugarcane, a highly polyploid crop speciesPlanta, 229
M. Davies (2003)
Singlet oxygen-mediated damage to proteins and its consequences.Biochemical and biophysical research communications, 305 3
G. Bartley, P. Scolnik (1995)
Plant carotenoids: pigments for photoprotection, visual attraction, and human health.The Plant cell, 7
L. Grivet, J. Glaszmann, M. Vincentz, Felipe Silva, P. Arruda (2002)
ESTs as a source for sequence polymorphism discovery in sugarcane: example of the Adh genesTheoretical and Applied Genetics, 106
S. Travella, Theres Klimm, B. Keller (2006)
RNA Interference-Based Gene Silencing as an Efficient Tool for Functional Genomics in Hexaploid Bread Wheat1[W][OA]Plant Physiology, 142
L. Grivet, P. Arruda (2002)
Sugarcane genomics: depicting the complex genome of an important tropical crop.Current opinion in plant biology, 5 2
M. Fromm, L. Taylor, V. Walbot (1985)
Expression of genes transferred into monocot and dicot plant cells by electroporation.Proceedings of the National Academy of Sciences of the United States of America, 82 17
Tao Wang, L. Iyer, R. Pancholy, Xiangyu Shi, T. Hall (2005)
Assessment of penetrance and expressivity of RNAi-mediated silencing of the Arabidopsis phytoene desaturase gene.The New phytologist, 167 3
H. Ma, Stefan Schulze, Suk-Ha Lee, M. Yang, E. Mirkov, James Irvine, Paul Moore, Andrew Paterson (2004)
An EST survey of the sugarcane transcriptomeTheoretical and Applied Genetics, 108
R. Birch (2007)
Metabolic Engineering in Sugarcane: Assisting the Transition to a Bio-based Economy, 9781402060311
G. Cordeiro, G. Taylor, R. Henry (2000)
Characterisation of microsatellite markers from sugarcane (Saccharum sp.), a highly polyploid species.Plant science : an international journal of experimental plant biology, 155 2
Richard Lawrence, C. Pikaard (2003)
Transgene-induced RNA interference: a strategy for overcoming gene redundancy in polyploids to generate loss-of-function mutations.The Plant journal : for cell and molecular biology, 36 1
L. Grivet, J. Glaszmann, P. Arruda (2001)
Sequence polymorphism from EST data in sugarcane: a fine analysis of 6-phosphogluconate dehydrogenase genesGenetics and Molecular Biology, 24
N. Smith, S. Singh, Ming-Bo Wang, P. Stoutjesdijk, A. Green, P. Waterhouse (2000)
Gene expression: Total silencing by intron-spliced hairpin RNAsNature, 407
S. Altschul, W. Gish, W. Miller, E. Myers, D. Lipman (1990)
Basic local alignment search tool.Journal of molecular biology, 215 3
C. McIntyre, C. McIntyre, Mark Jackson, Mark Jackson, G. Cordeiro, G. Cordeiro, O. Amouyal, O. Amouyal, S. Hermann, K. Aitken, K. Aitken, F. Eliott, F. Eliott, Robert Henry, Robert Henry, R. Casu, R. Casu, G. Bonnett, G. Bonnett (2006)
The identification and characterisation of alleles of sucrose phosphate synthase gene family III in sugarcaneMolecular Breeding, 18
F. Botha, B. Sawyer, R. Birch (2001)
Sucrose metabolism in the culm of transgenic sugarcane with reduced soluble acid invertase activity, 2
F. Papini-Terzi, F. Rocha, R. Vêncio, K. Oliveira, J. Felix, R. Vicentini, Cristiane Rocha, A. Simoes, E. Ulian, S. Mauro, A. Silva, C. Pereira, M. Menossi, G. Souza (2005)
Transcription profiling of signal transduction-related genes in sugarcane tissues.DNA research : an international journal for rapid publication of reports on genes and genomes, 12 1
S. Hansom, R. Bower, L. Zhang, B. Potier, A. Elliott, S. Basnayake, G. Codeiro, D. Hogarth, M. Cox, N. Berding, R. Birch (1999)
Regulation of transgene expression in sugarcane
D. Rossouw, S. Bosch, J. Kossmann, F. Botha, J. Groenewald (2007)
Downregulation of neutral invertase activity in sugarcane cell suspension cultures leads to a reduction in respiration and growth and an increase in sucrose accumulation.Functional plant biology : FPB, 34 6
J. Glaszmann, A. Fautret, J. Noyer, Philippe Feldmann, C. Lanaud (1989)
Biochemical genetic markers in sugarcaneTheoretical and Applied Genetics, 78
A. Christensen, R. Sharrock, P. Quail (1992)
Maize polyubiquitin genes: structure, thermal perturbation of expression and transcript splicing, and promoter activity following transfer to protoplasts by electroporationPlant Molecular Biology, 18
Hongmei Ma, H. Albert, R. Paull, P. Moore (2000)
Metabolic engineering of invertase activities in different subcellular compartments affects sucrose accumulation in sugarcane cellsAustralian Journal of Plant Physiology, 27
A. Vettore, Felipe Silva, E. Kemper, P. Arruda (2001)
The libraries that made SUCESTGenetics and Molecular Biology, 24
R. Verpoorte, A. Alfermann, T. Johnson (2007)
Applications of plant metabolic engineering
Sugarcane is a crop with great potential for metabolic engineering, but progress has been limited by highly efficient transgene silencing. The potential exists to utilize efficient gene silencing in molecular improvement through down-regulation of sugarcane genes. However, sugarcane is highly polyploid and heterozygous, which might complicate efforts to employ transgene-mediated silencing of endogenous genes. To explore this issue, we tested in sugarcane a construct designed for hairpin-mediated silencing of the phytoene desaturase (PDS) gene in sugarcane. The hairpin construct was designed based on the sugarcane PDS EST collection containing multiple alleles, to down-regulate the suite of PDS alleles. Three out of four plants transformed using the PDS hairpin construct showed detectable hairpin transcripts when analyzed by northern analysis, and displayed the photo-bleaching phenotype characteristic of PDS knockout lines. There was near-complete reduction in the levels of endogenous PDS transcripts in leaf tissue, indicating efficient hairpin-mediated down-regulation despite the highly polyploid and heterozygous sugarcane genome. Hairpin-mediated gene silencing should therefore be a powerful tool for the molecular improvement of this important crop.
Tropical Plant Biology – Springer Journals
Published: Nov 6, 2009
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.