Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Resonance assignments of bacteriophage T4 Y04L protein

Resonance assignments of bacteriophage T4 Y04L protein Phage study draws more attention recently as the bacterial antibiotic resistances become a major threat for global health. Bacteriophage T4 is one of the most studied the phages and the representative of Tevenvirinae subfamily. Since 1950s, T4 phage has been studied more intensively than any other large lytic phages and its biological studies have provided basis for current phage biology as well as other applications. However, among approximately 300 T4 genes, 130 of them still remain uncharacterized. Coded by y04L gene in pin-nrdC intergenic region, Y04L is an example of such proteins whose biological function and mechanism are yet to be addressed. While Pin blocks bacterial Lon protease and thus inhibits bacterial toxin–antitoxin system, NrdC or Glutaredoxin is a specific reducing agent for the phage-induced ribonucleotide reductase. With two interesting neighbouring genes, this 11.9 kDa protein may be functionally related to Pin or NrdC. Here, using solution-state NMR, our near-complete resonance assignment of Y04L provides a basis for future structure determination and further mechanism study. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Biomolecular NMR Assignments Springer Journals

Resonance assignments of bacteriophage T4 Y04L protein

Download PDF
4 pages

Loading next page...
 
/lp/springer-journals/resonance-assignments-of-bacteriophage-t4-y04l-protein-VsBc7ESgtf

References (16)

Publisher
Springer Journals
Copyright
Copyright © Springer Nature B.V. 2019
Subject
Physics; Biological and Medical Physics, Biophysics; Polymer Sciences; Biochemistry, general
ISSN
1874-2718
eISSN
1874-270X
DOI
10.1007/s12104-019-09919-5
Publisher site
See Article on Publisher Site

Abstract

Phage study draws more attention recently as the bacterial antibiotic resistances become a major threat for global health. Bacteriophage T4 is one of the most studied the phages and the representative of Tevenvirinae subfamily. Since 1950s, T4 phage has been studied more intensively than any other large lytic phages and its biological studies have provided basis for current phage biology as well as other applications. However, among approximately 300 T4 genes, 130 of them still remain uncharacterized. Coded by y04L gene in pin-nrdC intergenic region, Y04L is an example of such proteins whose biological function and mechanism are yet to be addressed. While Pin blocks bacterial Lon protease and thus inhibits bacterial toxin–antitoxin system, NrdC or Glutaredoxin is a specific reducing agent for the phage-induced ribonucleotide reductase. With two interesting neighbouring genes, this 11.9 kDa protein may be functionally related to Pin or NrdC. Here, using solution-state NMR, our near-complete resonance assignment of Y04L provides a basis for future structure determination and further mechanism study.

Journal

Biomolecular NMR Assignments – Springer Journals

Published: Apr 9, 2020

There are no references for this article.