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Relationship between the levels of serum high-sensitivity C-reactive protein and interleukin-6 and interleukin-6 gene polymorphisms in type 2 diabetes mellitus

Relationship between the levels of serum high-sensitivity C-reactive protein and interleukin-6... There has been growing evidence that low-grade activation of the immune system plays a role in the pathogenesis of insulin resistance and type 2 diabetes mellitus (T2DM). This study was based on the hypothesis that a single-nucleotide polymorphism in the promoter region of the interleukin-6 (IL-6) gene influences its transcription and thus affects serum levels of IL-6 and hence of the high-sensitivity C-reactive protein (hs-CRP). This may lead to insulin resistance and consequently type 2 diabetes. This study was performed on 60 subjects: 30 T2DM patients and 30 non-diabetic participants. For all subjects, the serum level of IL-6 was assayed by enzyme chemiluminescence, serum hs-CRP was assayed using a particle-enhanced immuno-turbidimetric assay and the IL-6 –174G/C gene polymorphism was detected by PCR-RFLP. There was no statistically significant association between the IL-6 –174G/C gene polymorphism and the presence of diabetes mellitus or with IL-6 or hs-CRP levels. The GG genotype was the most prevalent genotype in the participants studied and the presence of the C allele in the −174 position of the promoter region might be protective. However, the serum levels of IL-6 and hs-CRP were found to be higher in the diabetic than the non-diabetic group, reflecting the role played by both markers in the pathogenesis of T2DM. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Comparative Clinical Pathology Springer Journals

Relationship between the levels of serum high-sensitivity C-reactive protein and interleukin-6 and interleukin-6 gene polymorphisms in type 2 diabetes mellitus

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Publisher
Springer Journals
Copyright
Copyright © 2011 by Springer-Verlag London Limited
Subject
Medicine & Public Health; Pathology; Hematology; Oncology
eISSN
1618-565X
DOI
10.1007/s00580-011-1366-5
Publisher site
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Abstract

There has been growing evidence that low-grade activation of the immune system plays a role in the pathogenesis of insulin resistance and type 2 diabetes mellitus (T2DM). This study was based on the hypothesis that a single-nucleotide polymorphism in the promoter region of the interleukin-6 (IL-6) gene influences its transcription and thus affects serum levels of IL-6 and hence of the high-sensitivity C-reactive protein (hs-CRP). This may lead to insulin resistance and consequently type 2 diabetes. This study was performed on 60 subjects: 30 T2DM patients and 30 non-diabetic participants. For all subjects, the serum level of IL-6 was assayed by enzyme chemiluminescence, serum hs-CRP was assayed using a particle-enhanced immuno-turbidimetric assay and the IL-6 –174G/C gene polymorphism was detected by PCR-RFLP. There was no statistically significant association between the IL-6 –174G/C gene polymorphism and the presence of diabetes mellitus or with IL-6 or hs-CRP levels. The GG genotype was the most prevalent genotype in the participants studied and the presence of the C allele in the −174 position of the promoter region might be protective. However, the serum levels of IL-6 and hs-CRP were found to be higher in the diabetic than the non-diabetic group, reflecting the role played by both markers in the pathogenesis of T2DM.

Journal

Comparative Clinical Pathology – Springer Journals

Published: Nov 15, 2011

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