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- Publisher
- Springer Journals
- Copyright
- Copyright © 2006 by MAIK “Nauka/Interperiodica”
- Subject
- Life Sciences; Medical Microbiology; Biochemistry, general; Microbiology
- ISSN
- 0003-6838
- eISSN
- 1608-3024
- DOI
- 10.1134/S0003683806050024
- Publisher site
- See Article on Publisher Site
Abstract
The registration of the accumulation of polymerase chain reaction (PCR) products in the course of amplification (real-time PCR) requires specific equipment, i.e., detecting amplifiers capable of recording the level of fluorescence in the reaction tube during amplicon formation. When the time of the reaction is complete, researchers are able to obtain DNA accumulation graphs. This review discusses the most promising algorithms of the analysis of real-time PCR curves and possible errors, caused by the software used or by operators’ mistakes. The data included will assist researchers in understanding the features of a method to obtain more reliable results.
Journal
Applied Biochemistry and Microbiology – Springer Journals
Published: Aug 29, 2006