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Rapid determination of haemoglobin A1c and glucose in mice: Strain differences, glucose tolerance tests and the neonatal streptozotocin induced diabetic model

Rapid determination of haemoglobin A1c and glucose in mice: Strain differences, glucose tolerance... Blood glucose and Haemoglobin A1c (HbA1c) levels were evaluated from sequestrated blood samples from mice, using a combination of the Antsense II and DCA-2000 analysers, and using only 6μl whole blood. The difference between fed and fasted blood glucose and HbA1c concentrations among four strains of mice (inbred C57BL/6N, C3H/HeN, hybrid B6C3F1 and outbred CD-1) was examined, and glucose tolerance was further evaluated in each strain by an intraperitoneal glucose tolerance test (IPGTT) using this apparatus. There was considerable variation between fed and fasted glucose and HbA1c concentrations, with C3H/HeN mice being the most glucose tolerant and CD-1 mice the least glucose tolerant. These findings did not contradict previous observations. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Comparative Clinical Pathology Springer Journals

Rapid determination of haemoglobin A1c and glucose in mice: Strain differences, glucose tolerance tests and the neonatal streptozotocin induced diabetic model

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Publisher
Springer Journals
Copyright
Copyright © 1998 by Springer-Verlag London Limited
Subject
Medicine & Public Health; Hematology; Oncology; Pathology
eISSN
1433-2973
DOI
10.1007/BF02628106
Publisher site
See Article on Publisher Site

Abstract

Blood glucose and Haemoglobin A1c (HbA1c) levels were evaluated from sequestrated blood samples from mice, using a combination of the Antsense II and DCA-2000 analysers, and using only 6μl whole blood. The difference between fed and fasted blood glucose and HbA1c concentrations among four strains of mice (inbred C57BL/6N, C3H/HeN, hybrid B6C3F1 and outbred CD-1) was examined, and glucose tolerance was further evaluated in each strain by an intraperitoneal glucose tolerance test (IPGTT) using this apparatus. There was considerable variation between fed and fasted glucose and HbA1c concentrations, with C3H/HeN mice being the most glucose tolerant and CD-1 mice the least glucose tolerant. These findings did not contradict previous observations.

Journal

Comparative Clinical Pathology – Springer Journals

Published: May 3, 2007

References

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