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J. Vos, J. Buys, P. Beekhof, A. Hagenaars (1979)
QUANTIFICATION OF TOTAL IgM AND IgG AND SPECIFIC IgM AND IgG TO A THYMUS‐INDEPENDENT (LPS) AND A THYMUS‐DEPENDENT (TETANUS TOXOID) ANTIGEN IN THE RAT BY ENZYME‐LINKED IMMUNOSORBENT ASSAY (ELISA)Annals of the New York Academy of Sciences, 320
H Bazin (1990)
Immunologic animale
M. Castell, M. Gisbert, J. Queralt, C. Castellote (1987)
ELISA for total IgE and IgG detection in rat serum. Changes with immunization or adjuvants.Allergologia et immunopathologia, 15 1
JG Vos, J Buys, P Beekhof (1979)
Quantification of total IgM and IgG to thymus-independent (LPS) and a thymus dependent (Tetanus toxoid) antigen in the rat by enzyme-linked immunosorbent assay (ELISA)Ann NY Acad Sci, 320
H. Bazin, A. Beckers, G. Urbain-Vansanten, R. Pauwels, C. Bruyns, A. Tilkin, B. Platteau, J. Urbain (1978)
Transplantable IgD immunoglobulin-secreting tumors in rat.Journal of immunology, 121 5
J. Salgado, A. Gilabert, M. Castell, C. Castellote, J. Queralt (1988)
ELISA for quantification of specific IgG and IgE antibodies to ovalbumin.Allergologia et immunopathologia, 16 2
C. Davis, M. Cohen, M. Anderson, J. Reinarz, M. Warren (1987)
Total and specific immunoglobulin response to acute and chronic urinary tract infections in a rat model.The Journal of urology, 138 5
H. Bazin, A. Beckers, P. Querinjean (1974)
Three classes and four (sub)classes of rat immunoglobulins: IgM, IgA, Ige and IgG1, IgG2a, IgG2b, IgG2cEuropean Journal of Immunology, 4
H Bazin, A Beckers, G Urbain-Vansanten (1978)
Transplantable IgD immunoglobulin-secreting tumors in ratsJ Imunol, 121
(1990)
Immunologic des rogeurs
Routine determination of IgG and IgM levels is likely to be included in future international immunotoxicity assessment guidelines with the rat as the required animal species. Only a few historical data are available on these two parameters in the rat. We describe a quantitative ELISA sandwich method using commercially available purified rat immunoglobulin as standards and provide the normal range for IgG and IgM values in healthy untreated 10 ± 2 to 30 ± 3 weekold Sprague-Dawley rats. Our data indicate that both IgM and IgG levels increase gradually with age. In addition, we determined IgG values in rats hyperimmunised with protamine, lysozyme and casein.
Comparative Clinical Pathology – Springer Journals
Published: Sep 8, 2004
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