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- Publisher
- Springer Journals
- Copyright
- Copyright © 2012 by Pleiades Publishing, Ltd.
- Subject
- Life Sciences; Medical Microbiology; Biochemistry, general; Microbiology
- ISSN
- 0003-6838
- eISSN
- 1608-3024
- DOI
- 10.1134/S0003683812070058
- Publisher site
- See Article on Publisher Site
Abstract
A cell surface display system has been developed in the yeast Yarrowia lipolytica using the cell wall protein YlPir1. The red fluorescent protein TurboFP635 was used as a model protein. The expression plasmid in which the YlPIR1 gene without stop-codon was fused in-frame with the nucleotide sequence encoding TurboFP635 was constructed. The thus obtained recombinant protein complex consists of the YlPir1 the C-terminus of which is fused to the N-terminus of red fluorescent protein. Cell surface display of TurboFP635 was confirmed by fluorescent microscopy. This N-terminal system of protein incorporation in the cell wall could be efficiently applied to the cell surface display of enzymes with active sites located near the C-terminus, for example, lipases.
Journal
Applied Biochemistry and Microbiology – Springer Journals
Published: Oct 24, 2012