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(1993)
Clonal and long-term cultures using human bone marrow. In: Testa NG, Molineux G (eds) Haemopoiesis, a practical approach
Ron Caspi, R. Avtalion (1984)
Evidence for the existence of an IL-2-like lymphocyte growth promoting factor in a bony fish, Cyprinus carpio.Developmental and comparative immunology, 8 1
L. Haynes, N. Cohen
Reprints Available Directly from the Publisher Photocopying Permitted by License Only Further Characterization of an Interleukin-2-1ike Cytokine Produced by Xenopus Laevis T Lymphocytes
CM Heyworth, E Spooncer (1993)
Haemopoiesis, a practical approach
M. Diago, M. López-Fierro, B. Razquin, A. Villena (1993)
Long-term myelopoietic cultures from the renal hematopoietic tissue of the rainbow trout, Oncorhynchus mykiss W.: phenotypic characterization of the stromal cells.Experimental hematology, 21 9
RR Caspi, RR Avtalion (1984)
Evidence for the existence of an Il-2 like lymphocyte growth factor in a boney fish,Cyprinus carpioDev Comp Immunol, 8
V. Kotnik, W. Fleischmann (1990)
A simple and rapid method to determine hematopoietic growth factor activity.Journal of immunological methods, 129 1
(1993)
In vitro clonal assays for murine multipotential and lineage restricted myeloid progenitor cells
(1990)
Do fish lymphocyte secrete interferon y ?
(1993)
Clonal and long - term cultures using human bone marrow
Gwyn Williams, Christopher Smith, E. Spooncer, T. Dexter, Dale Taylor (1990)
Haemopoietic colony stimulating factors promote cell survival by suppressing apoptosisNature, 343
M. Gordon, D. Clarke, J. Atkinson, M. Greaves (1990)
Hemopoietic progenitor cell binding to the stromal microenvironment in vitro.Experimental hematology, 18 7
H. Mayani, L. Guilbert, A. Janowska-Wieczorek (1992)
Biology of the hemopoietic microenvironmentEuropean Journal of Haematology, 49
(1985)
Early steps of lymphocyte activation bypassed by calcium ionophore and phorbolester acting in synergy
N. Roehm, G. Rodgers, S. Hatfield, A. Glasebrook (1991)
An improved colorimetric assay for cell proliferation and viability utilizing the tetrazolium salt XTT.Journal of immunological methods, 142 2
G. Warr, R. Simon (1983)
The mitogen response potential of lymphocytes from the rainbow trout (Salmo gairdneri) re-examined.Developmental and comparative immunology, 7 2
Guilan Lin, C. Ellsaesser, L. Clem, Norman Miller (1992)
Phorbol ester/calcium ionophore activate fish leukocytes and induce long-term cultures.Developmental and comparative immunology, 16 2-3
E. Siegl, S. Albrecht, B. Lüdtke (1993)
Long-term liquid culture of haematopoietic precursor cells from the head kidney and spleen of the rainbow trout (Oncorhynchus mykiss)Comparative Haematology International, 3
M. Owen (1988)
Marrow stromal stem cellsJournal of Cell Science, 1988
C. Bürger, M. Wick, S. Brüsselbach, R. Müller (1994)
Differential induction of 'metabolic genes' after mitogen stimulation and during normal cell cycle progression.Journal of cell science, 107 ( Pt 1)
T Morimoto, A Itoum, I Yamaguchi (1993)
In vitro colony formation of carp granulocytes in semi solid agar culture systemGyobyo Kenyu, 28
M. Lloret, L. Ramírez, A. Ramírez, J. Preciado (1992)
Macrophage colony-stimulating factor enhances the respiratory burst of human monocytes in response to Entamoeba histolytica.Archives of medical research, 23 2
ML Diago, MP Lopezfierro, B Razquin (1993)
Long term myelopoietic cultures from the renal hematopoietic tissue of the rainbow trout,Oncorhynchus mykiss WExp Hematol, 21
R. Caspi, R. Shahrabani, T. Kehati-Dan, R. Avtalion (1984)
Heterogeneity of mitogen-responsive lymphocytes in carp (Cyprinus carpio).Developmental and comparative immunology, 8 1
S. Rosenberg-Wiser, R. Avtalion (1982)
The cells involved in the immune response of fish. III. Culture requirements of PHA-stimulated carp (Cyprinus carpio) lymphocytes.Developmental and comparative immunology, 6 4
S. Graham, C. Secombes (1990)
Do fish lymphocytes secrete interferon‐γ?Journal of Fish Biology, 36
L Haynes, N Cohen (1993)
Further characterization of an interleukin-2-like cytokine produced byXenopus laevis T lymphocytesDev Immunol, 3
(1988)
Marrow stromal cells
E. Wynter, T. Allen, L. Coutinho, D. Flavell, S. Flavell, T. Dexter (1993)
Localisation of granulocyte macrophage colony-stimulating factor in human long-term bone marrow cultures. Biological and immunocytochemical characterisation.Journal of cell science, 106 ( Pt 3)
J. Dekoning, S. Kaattari (1991)
Mitogenesis of rainbow trout peripheral blood lymphocytes requires homologous plasma for optimal responsivenessIn Vitro Cellular & Developmental Biology - Animal, 27
D. Tillitt, J. Giesy, P. Fromm (1988)
In vitro mitogenesis of peripheral blood lymphocytes from rainbow trout (Salmo gairdneri).Comparative biochemistry and physiology. A, Comparative physiology, 89 1
(1993)
In vitro clonal assays for murine multipotential and lineage restricted myeloid progenitor cells. In: Testa NG, Molineux G (eds) Haemopoiesis, a practical approach
C. Secombes (1987)
Lymphokine-release from rainbow trout leucocytes stimulated with concanavalin A. Effects upon macrophage spreading and adherence.Developmental and comparative immunology, 11 3
J. Grondel, E. Harmsen (1984)
Phylogeny of interleukins: growth factors produced by leucocytes of the cyprinid fish, Cyprinus carpio L.Immunology, 52 3
H Kodama, M Mukamoto, T Baba (1994)
Modulators of fish immune response
L. Hardie, L. Chappell, C. Secombes (1994)
Human tumor necrosis factor α influences rainbow trout Oncorhynchus mykiss leucocyte responsesVeterinary Immunology and Immunopathology, 40
(1992)
Janowskawieczorek A (1992) Biology of hemopoietic microenvironment
A. Zuasti, Concepción Ferrer (1988)
Haemopoiesis in the head kidney of Sparus auratus.Archives of histology and cytology, 52 3
A. Truneh, F. Albert, P. Golstein, A. Schmitt-Verhulst (1985)
Early steps of lymphocyte activation bypassed by synergy between calcium ionophores and phorbol esterNature, 313
T. Moritomo, A. Itou, I. Yamaguchi, Tasuku Watanabe (1993)
In Vitro Colony Formation of Carp Granulocytes in a Semi-Solid Agar Culture SystemFish Pathology, 28
R. Memon, K. Feingold, C. Grunfeld (1994)
The Effects of Cytokines on Intermediary MetabolismEndocrinologist, 4
(1994)
Macrophage-colony stimulating response, 1. In: Modulators of fish immune response
Supernatants or conditioned media (CM) were produced by rainbow trout pronephros cells (PNC) and spleen cell cultures (1×106/ml), by addition of 20 μg/ml phythaemagglutinin (PHA), 5 ng/ml 12-O-tetradecanoyl-phorbol-13-acetate (PHA) (for 2h), PHA together with PMA, 10% horse serum (HS), or 100 μg/ml concanavalin A (ConA) after a culture of 7 days. Only PNC were effective growth factor producers. The effect of different concentrations of CM (5%–28%) on cell number was tested after a cultivation period of 14 days. PNC at a concentration of 1×106/ml were cultured with various concentrations of CM and the proliferation was tested by the XTT-test (testing the dehydrogenase activity of the cells by formation of a formazan) after 10 days. CM produced by cells with PHA, PHA and PMA and HS increased the proliferation in a concentration-dependent manner. CM produced with PMA alone was effective in the XTT-test. There was no synergistic or additive effect of PMA with PHA. CM produced with ConA had no effect, although in the XTT-test a strong proliferation of PNC in presence of 100 μg/ml ConA was observed. In a semisolid culture with collagen, CM treatment resulted in prevention of cell death and an increase in cell size but did not induce proliferation. All CM obtained from spleen cells had no effect. Stimulation of spleen cells by CM could be seen only in the XTT-test. PHA and HS trigger the PNC to release growth factors in vitro which stimulate cell growth and/or prevent cell death.
Comparative Clinical Pathology – Springer Journals
Published: Jul 21, 2005
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