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Preparation and cytotoxicity comparison of type a gelatin nanoparticles with recombinant human gelatin nanoparticles

Preparation and cytotoxicity comparison of type a gelatin nanoparticles with recombinant human... Abstract Gelatin nanoparticles derived from bovine or porcine have been developed as various types of drug delivery system, and they need to be cross-linked to maintain their physicochemical properties in aqueous environments. Although gelatin is a widely used material in pharmaceutical industries, the safety issue of animal-origin gelatins, such as transmissible mad cow disease and anaphylaxis, remains to be solved. The purpose of this study was to prepare type A gelatin (GA) nanoparticles by modified, two-step, desolvation method and compare the toxicity of the resulting GA nanoparticles with recombinant human gelatin (rHG) nanoparticles. The GA nanoparticles were characterized, and drug loading and release pattern were measured. FITC-BSA, a model protein, was efficiently loaded in the nanoparticles and then released in a biphasic and sustained release pattern without an initial burst. In particular, the cell viability of the GA nanoparticles was less than that of the rHG nanoparticles. This finding suggests that rHG nanoparticles should be considered as an alternative to animal-origin gelatin nanoparticles in order to minimize the safety problems. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png "Macromolecular Research" Springer Journals

Preparation and cytotoxicity comparison of type a gelatin nanoparticles with recombinant human gelatin nanoparticles

Won, Young-Wook; Kim, Yong-Hee
"Macromolecular Research" , Volume 17 (7): 5 – Jul 1, 2009
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References (16)

Publisher
Springer Journals
Copyright
2009 The Polymer Society of Korea and Springer
ISSN
1598-5032
eISSN
2092-7673
DOI
10.1007/BF03218893
Publisher site
See Article on Publisher Site

Abstract

Abstract Gelatin nanoparticles derived from bovine or porcine have been developed as various types of drug delivery system, and they need to be cross-linked to maintain their physicochemical properties in aqueous environments. Although gelatin is a widely used material in pharmaceutical industries, the safety issue of animal-origin gelatins, such as transmissible mad cow disease and anaphylaxis, remains to be solved. The purpose of this study was to prepare type A gelatin (GA) nanoparticles by modified, two-step, desolvation method and compare the toxicity of the resulting GA nanoparticles with recombinant human gelatin (rHG) nanoparticles. The GA nanoparticles were characterized, and drug loading and release pattern were measured. FITC-BSA, a model protein, was efficiently loaded in the nanoparticles and then released in a biphasic and sustained release pattern without an initial burst. In particular, the cell viability of the GA nanoparticles was less than that of the rHG nanoparticles. This finding suggests that rHG nanoparticles should be considered as an alternative to animal-origin gelatin nanoparticles in order to minimize the safety problems.

Journal

"Macromolecular Research" – Springer Journals

Published: Jul 1, 2009

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