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Abstract Novel porous scaffolds composed of gelatin/κ-carrageenan (GC) were fabricated by freeze-drying followed by chemical cross-linking with 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC). The morphology of the insoluble GC sponges was examined by field emission scanning electron microscopy (FE-SEM). The porosity of the GC sponge increased with increasing kappa-carrageenan content. Implantation of a GC sponge into the subcutaneous connective tissue of Wistar rats confirmed that the scaffold was biodegradable. Fibroblasts infiltrated into the sponge matrix, and regenerated collagen in the matrix to a level of 25% at 14 days after surgery. The C-reactive protein level in the blood samples also showed a similar result. The blood and histological results show that the GC sponges have good biocompatibility and low antigenicity indicating that they would be safe and effective tissue engineering scaffolds.
"Macromolecular Research" – Springer Journals
Published: Jan 1, 2010
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