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Pregnan X Receptor Functioning under Conditions of Nitrosative Stress

Pregnan X Receptor Functioning under Conditions of Nitrosative Stress —Pregnan X receptor (PXR) is a nuclear receptor that plays an important role in the regulation of the expression enzymes of biotransformation and metabolism. The functioning and possible mechanisms of PXR regulation under conditions of nitrosative stress have not been studied yet and this was the purpose of this study. Nitrosative stress (NS) was modeled in Caco-2 cells, which were incubated in the presence of 1 μM, 10 μM, 50 μM, 100 μM, and 500 μM concentrations of S-nitrosoglutathione (GSNO) for 3 h, 24 h, and 72 h. The amount of PXR was assessed by Western blotting. Incubation of Caco-2 cells with all GSNO concentrations for 3 h led to a decrease in the amount of PXR. Incubation with GSNO (1−50 μM) for 24 h was accompanied by an increase in the amount of PXR, while at a higher concentration (100 μM) this indicator did not significantly differ from the control and at a concentration of 500 μM it was below the control level. Prolonged incubation (for 72 h) enhanced NS and led to a normalization (1 μM GSNO) or a decrease of the PXR level (10−500 μM GSNO). Bityrosine formed during NS induced PXR expression at concentrations of 0.4 mM and 1 mM. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png "Biochemistry (Moscow), Supplement Series B:Biomedical Chemistry" Springer Journals

Pregnan X Receptor Functioning under Conditions of Nitrosative Stress

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References (32)

Publisher
Springer Journals
Copyright
Copyright © Pleiades Publishing, Ltd. 2022. ISSN 1990-7508, Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry, 2022, Vol. 16, No. 2, pp. 140–147. © Pleiades Publishing, Ltd., 2022. Russian Text © The Author(s), 2021, published in Biomeditsinskaya Khimiya.
ISSN
1990-7508
eISSN
1990-7516
DOI
10.1134/s1990750822020020
Publisher site
See Article on Publisher Site

Abstract

—Pregnan X receptor (PXR) is a nuclear receptor that plays an important role in the regulation of the expression enzymes of biotransformation and metabolism. The functioning and possible mechanisms of PXR regulation under conditions of nitrosative stress have not been studied yet and this was the purpose of this study. Nitrosative stress (NS) was modeled in Caco-2 cells, which were incubated in the presence of 1 μM, 10 μM, 50 μM, 100 μM, and 500 μM concentrations of S-nitrosoglutathione (GSNO) for 3 h, 24 h, and 72 h. The amount of PXR was assessed by Western blotting. Incubation of Caco-2 cells with all GSNO concentrations for 3 h led to a decrease in the amount of PXR. Incubation with GSNO (1−50 μM) for 24 h was accompanied by an increase in the amount of PXR, while at a higher concentration (100 μM) this indicator did not significantly differ from the control and at a concentration of 500 μM it was below the control level. Prolonged incubation (for 72 h) enhanced NS and led to a normalization (1 μM GSNO) or a decrease of the PXR level (10−500 μM GSNO). Bityrosine formed during NS induced PXR expression at concentrations of 0.4 mM and 1 mM.

Journal

"Biochemistry (Moscow), Supplement Series B:Biomedical Chemistry" – Springer Journals

Published: Jun 1, 2022

Keywords: pregnan X receptor; nitrosative stress; nitrosoglutathione; Caco-2 cell line

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