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Physiological and biochemical parameters influencing ex vitro establishment of the in vitro regenerants of Albizia lebbeck (L.) Benth.: an important soil reclaiming plantation tree

Physiological and biochemical parameters influencing ex vitro establishment of the in vitro... Present approach demonstrates a speedy and competent in vitro regeneration method for A. lebbeck . The hypocotyl explants (HP) excised from a 15-day-old seedlings were cultured on Murashige and Skoog (MS) medium supplemented with different concentrations (0.5, 2.5, 5.0, 7.5, 10.0 and 12.5 μM) of 6-Benzyladenine (BA), Kinetin (Kn) and 2-Isopentenyl adenine (2-iP) singly as well as in combination with α-naphthalene acetic acid (NAA) or Indole-3-butyric acid (IBA) or Indole-3-acetic acid (IAA; 0.1, 0.5, 1.0, 1.5 and 2.0 μM). The best shoot regeneration rate (88 %) with number of shoots (34.00 ± 1.15) and shoot length (6.30 ± 0.05 cm) per explant was obtained on MS + BA (7.5 μM) + NAA (0.5 μM) after 8 weeks of culture. While, the explants cultured onto the MS + TDZ (Thidiazuron; 0.5, 1.0, 2.5, 5.0 and 7.5 μM) singly showed maximum shoot proliferation response of 77 % when, TDZ exposed cultures were transferred on the BA (7.5 µM) + NAA (0.5 µM) supplied MS medium producing maximum no. of shoots (20.30 ± 0.32) and shoot length (5.15 ± 0.08 cm) after 8 weeks of culture. The regenerated shoots were rooted by using ex vitro rooting method and successfully acclimatized by using a simple acclimatization procedure of 70 days, primarily in soilrite for 28 days and finally in soilrite + garden soil (1:1) for 70 days under culture room conditions (150 PPFD). This acclimatization period under the optimized conditions of culture room ensures a high survival rate (80 %) of the plantlets in the field conditions. Photosynthetic pigments (Chlorophyll a, b and carotenoid content) evaluated during acclimatization period showed a decreasing trend in the initial 14 days afterwards a significant increase in the subsequent period of 21–70 days. While all the stress parameters MDA and H 2 O 2 content showed decreasing trend all through the acclimatization, concurrently all the tested antioxidative enzymes (SOD, CAT, APX and GR) exhibited an increasing trend. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Agroforestry Systems Springer Journals

Physiological and biochemical parameters influencing ex vitro establishment of the in vitro regenerants of Albizia lebbeck (L.) Benth.: an important soil reclaiming plantation tree

Agroforestry Systems , Volume 89 (4) – Aug 1, 2015

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References (66)

Publisher
Springer Journals
Copyright
Copyright © 2015 by Springer Science+Business Media Dordrecht
Subject
Life Sciences; Forestry; Agriculture
ISSN
0167-4366
eISSN
1572-9680
DOI
10.1007/s10457-015-9809-7
Publisher site
See Article on Publisher Site

Abstract

Present approach demonstrates a speedy and competent in vitro regeneration method for A. lebbeck . The hypocotyl explants (HP) excised from a 15-day-old seedlings were cultured on Murashige and Skoog (MS) medium supplemented with different concentrations (0.5, 2.5, 5.0, 7.5, 10.0 and 12.5 μM) of 6-Benzyladenine (BA), Kinetin (Kn) and 2-Isopentenyl adenine (2-iP) singly as well as in combination with α-naphthalene acetic acid (NAA) or Indole-3-butyric acid (IBA) or Indole-3-acetic acid (IAA; 0.1, 0.5, 1.0, 1.5 and 2.0 μM). The best shoot regeneration rate (88 %) with number of shoots (34.00 ± 1.15) and shoot length (6.30 ± 0.05 cm) per explant was obtained on MS + BA (7.5 μM) + NAA (0.5 μM) after 8 weeks of culture. While, the explants cultured onto the MS + TDZ (Thidiazuron; 0.5, 1.0, 2.5, 5.0 and 7.5 μM) singly showed maximum shoot proliferation response of 77 % when, TDZ exposed cultures were transferred on the BA (7.5 µM) + NAA (0.5 µM) supplied MS medium producing maximum no. of shoots (20.30 ± 0.32) and shoot length (5.15 ± 0.08 cm) after 8 weeks of culture. The regenerated shoots were rooted by using ex vitro rooting method and successfully acclimatized by using a simple acclimatization procedure of 70 days, primarily in soilrite for 28 days and finally in soilrite + garden soil (1:1) for 70 days under culture room conditions (150 PPFD). This acclimatization period under the optimized conditions of culture room ensures a high survival rate (80 %) of the plantlets in the field conditions. Photosynthetic pigments (Chlorophyll a, b and carotenoid content) evaluated during acclimatization period showed a decreasing trend in the initial 14 days afterwards a significant increase in the subsequent period of 21–70 days. While all the stress parameters MDA and H 2 O 2 content showed decreasing trend all through the acclimatization, concurrently all the tested antioxidative enzymes (SOD, CAT, APX and GR) exhibited an increasing trend.

Journal

Agroforestry SystemsSpringer Journals

Published: Aug 1, 2015

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