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Physico-chemical Properties and In vitro Cellular Response of Electrospun Polyurethane Nanofibers Enriched with Copper Chloride and Natural Fish Head Powder

Physico-chemical Properties and In vitro Cellular Response of Electrospun Polyurethane Nanofibers... Tissue engineering strategies include successful management of bone injuries with biomaterials to develop bone-like structures. The production of natural biomaterials for this purpose helps both to ensure tissue integrity and to present alternative products for clinical purposes. However, natural materials are mostly used together with synthetic or metallic materials due to their low stability and mechanical properties for bone tissue regeneration. There is a clinical need for material combinations that have both antimicrobial properties and the ability to increase osteogenic induction. This work aims to fabricate a nanofiber scaffold using polyurethane (PU) loaded with natural fish head powder (FHP) from Argyrosomus regius species and copper (II) chloride (CuCl2) using the electrospinning technique in order to respond to this clinical need. There has not been any material combination in the literature that contains these compounds, supports bone differentiation of stem cells, and prevents bacterial adhesion. To evaluate our composite materials morphology, chemical structure, wettability, and thermal analysis were studied with different techniques. Anti-adhesive properties of the Streptococcus mitis (S. mitis) on composite materials were tested with bacterial colonization, and osteogenic inductive properties by human bone marrow mesenchymal stem cells (hBMSCs) with and without osteogenic differentiation medium were performed with cell viability assay, Alizarin Red staining, Ca deposition and SEM analysis. According to FESEM-EDS and ATR_FTIR data, the additives were highly incorporated into the PU nanofibers. The produced materials had randomly oriented nanofiber with a porosity of ∼72 %. The FHP loaded nanofiber had an average diameter of ∼185 nm. The swelling behavior of FHP-loaded PU roughly decreased for 3, 5, and 7 days due to the hydrophobic character of the sample. An increasing percentage of FHP decreased the bacterial colonization of S. mitis. PUn-CuCl2-FHP nanofibers did not exhibit any cytotoxic effect on the hBMSCs. According to Alizarin Red Staining and Ca deposition analysis, stimulation with both the material and the osteogenic medium triggered the bone differentiation of the hBMSCs in a combined manner at days-14. Based on the results, it can be deduced that natural fish head is a promising additive material for fabricating bone-like biomaterials. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Fibers and Polymers Springer Journals

Physico-chemical Properties and In vitro Cellular Response of Electrospun Polyurethane Nanofibers Enriched with Copper Chloride and Natural Fish Head Powder

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References (61)

Publisher
Springer Journals
Copyright
Copyright © The Korean Fiber Society 2022
ISSN
1229-9197
eISSN
1875-0052
DOI
10.1007/s12221-022-0404-z
Publisher site
See Article on Publisher Site

Abstract

Tissue engineering strategies include successful management of bone injuries with biomaterials to develop bone-like structures. The production of natural biomaterials for this purpose helps both to ensure tissue integrity and to present alternative products for clinical purposes. However, natural materials are mostly used together with synthetic or metallic materials due to their low stability and mechanical properties for bone tissue regeneration. There is a clinical need for material combinations that have both antimicrobial properties and the ability to increase osteogenic induction. This work aims to fabricate a nanofiber scaffold using polyurethane (PU) loaded with natural fish head powder (FHP) from Argyrosomus regius species and copper (II) chloride (CuCl2) using the electrospinning technique in order to respond to this clinical need. There has not been any material combination in the literature that contains these compounds, supports bone differentiation of stem cells, and prevents bacterial adhesion. To evaluate our composite materials morphology, chemical structure, wettability, and thermal analysis were studied with different techniques. Anti-adhesive properties of the Streptococcus mitis (S. mitis) on composite materials were tested with bacterial colonization, and osteogenic inductive properties by human bone marrow mesenchymal stem cells (hBMSCs) with and without osteogenic differentiation medium were performed with cell viability assay, Alizarin Red staining, Ca deposition and SEM analysis. According to FESEM-EDS and ATR_FTIR data, the additives were highly incorporated into the PU nanofibers. The produced materials had randomly oriented nanofiber with a porosity of ∼72 %. The FHP loaded nanofiber had an average diameter of ∼185 nm. The swelling behavior of FHP-loaded PU roughly decreased for 3, 5, and 7 days due to the hydrophobic character of the sample. An increasing percentage of FHP decreased the bacterial colonization of S. mitis. PUn-CuCl2-FHP nanofibers did not exhibit any cytotoxic effect on the hBMSCs. According to Alizarin Red Staining and Ca deposition analysis, stimulation with both the material and the osteogenic medium triggered the bone differentiation of the hBMSCs in a combined manner at days-14. Based on the results, it can be deduced that natural fish head is a promising additive material for fabricating bone-like biomaterials.

Journal

Fibers and PolymersSpringer Journals

Published: Nov 1, 2022

Keywords: Polyurethane; Fish head powder; CuCl2; Osteogenic differentiation; Streptococcus mitis

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