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Origin of hydrogen peroxide during the cowpea- Xanthomonas interaction

Origin of hydrogen peroxide during the cowpea- Xanthomonas interaction To assay hydrogen peroxide (H2O2) production by phytopathogenic bacteria in vivo, cowpea seedlings were inoculated using wild-type Xanthomonas campestris pv. phaseoli and its mutant strain on ahpC (with attenuated H2O2 production). Antibiotic rifampicin (Rif) was used to kill off the Xanthomonas campestris pv. phaseoli in cowpea leaves. The optimal concentration of Rif to eradicate the bacteria was 500 μg mL–1, and duration of the Rif treatment to eradicate the bacteria in the cowpea tissues was 48 h. This level of Rif did not significantly affect chloroplast and mitochondrial function in cowpea leaves. The concentration of H2O2 in cowpea inoculated with wild-type or mutant strain of Xanthomonas campestris pv. phaseoli following Rif treatment was measured. Increased H2O2 accumulation was observed in cowpea leaves respectively inoculated with wild-type and mutant strain. The accumulation of excess H2O2 was significantly decreased by the Rif treatment on cowpea wild-type bacteria systems. However, the level of H2O2 in cowpea leaves inoculated with mutant strains following Rif treatment was at the same level as in plant leaves without Rif treatment. The results demonstrate that the change in H2O2 concentration observed in vivo was caused by wild-type bacteria. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Australasian Plant Pathology Springer Journals

Origin of hydrogen peroxide during the cowpea- Xanthomonas interaction

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References (48)

Publisher
Springer Journals
Copyright
Copyright © 2011 by Australasian Plant Pathology Society Inc.
Subject
Life Sciences; Entomology; Plant Pathology; Ecology; Plant Sciences; Agriculture
ISSN
0815-3191
eISSN
1448-6032
DOI
10.1007/s13313-011-0080-8
Publisher site
See Article on Publisher Site

Abstract

To assay hydrogen peroxide (H2O2) production by phytopathogenic bacteria in vivo, cowpea seedlings were inoculated using wild-type Xanthomonas campestris pv. phaseoli and its mutant strain on ahpC (with attenuated H2O2 production). Antibiotic rifampicin (Rif) was used to kill off the Xanthomonas campestris pv. phaseoli in cowpea leaves. The optimal concentration of Rif to eradicate the bacteria was 500 μg mL–1, and duration of the Rif treatment to eradicate the bacteria in the cowpea tissues was 48 h. This level of Rif did not significantly affect chloroplast and mitochondrial function in cowpea leaves. The concentration of H2O2 in cowpea inoculated with wild-type or mutant strain of Xanthomonas campestris pv. phaseoli following Rif treatment was measured. Increased H2O2 accumulation was observed in cowpea leaves respectively inoculated with wild-type and mutant strain. The accumulation of excess H2O2 was significantly decreased by the Rif treatment on cowpea wild-type bacteria systems. However, the level of H2O2 in cowpea leaves inoculated with mutant strains following Rif treatment was at the same level as in plant leaves without Rif treatment. The results demonstrate that the change in H2O2 concentration observed in vivo was caused by wild-type bacteria.

Journal

Australasian Plant PathologySpringer Journals

Published: Oct 6, 2011

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