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Optimization of the Method of Obtaining 9α-Hydroxy-androst-4-ene-3,17-dione—the Key Intermediate in the Synthesis of Highly Active Fluorinated Corticosteroids from Phytosterols

Optimization of the Method of Obtaining 9α-Hydroxy-androst-4-ene-3,17-dione—the Key Intermediate... ISSN 0003-6838, Applied Biochemistry and Microbiology, 2019, Vol. 55, No. 1, pp. 37–40. © Pleiades Publishing, Inc., 2019. Russian Text © N.V. Karpova, T.S. Stytsenko, V.V. Yaderets, V.A. Andryushina, V.V. Dzhavakhiya, 2019, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2019, Vol. 55, No. 1, pp. 51–55. Optimization of the Method of Obtaining 9α-Hydroxy-androst-4-ene-3,17-dione—the Key Intermediate in the Synthesis of Highly Active Fluorinated Corticosteroids from Phytosterols a a a a, a N. V. Karpova , T. S. Stytsenko , V. V. Yaderets , V. A. Andryushina *, and V. V. Dzhavakhiya Bioengineering Institute, Biotechnology Research Center, Russian Academy of Sciences, Moscow, 119071 Russia *e-mail: andryushina@rambler.ru Received April 13, 2018; revised July 16, 2018; accepted July 25, 2018 Abstract—A method of phytosterol conversion into 9α-hydroxy-androstenedione under high loads of ste- roid substrate has been developed with the use of mixed cultures of the actinobacteria M. neoaurum and R. erythropolis. The introduction of a 9α-hydroxylating culture after the first 70 h of conversion made it possible to increase process selectivity and to exclude the use of methylcyclodextrin, which is expensive and diff icult to regenerate. Keywords: phytosterols, mixed cultures, 9α-hydroxylation, f luorinated corticosteroids DOI: 10.1134/S0003683819010071 INTRODUCTION 11-hydroxylation from the production process due to http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Applied Biochemistry and Microbiology Springer Journals

Optimization of the Method of Obtaining 9α-Hydroxy-androst-4-ene-3,17-dione—the Key Intermediate in the Synthesis of Highly Active Fluorinated Corticosteroids from Phytosterols

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Publisher
Springer Journals
Copyright
Copyright © 2019 by Pleiades Publishing, Inc.
Subject
Life Sciences; Biochemistry, general; Microbiology; Medical Microbiology
ISSN
0003-6838
eISSN
1608-3024
DOI
10.1134/S0003683819010071
Publisher site
See Article on Publisher Site

Abstract

ISSN 0003-6838, Applied Biochemistry and Microbiology, 2019, Vol. 55, No. 1, pp. 37–40. © Pleiades Publishing, Inc., 2019. Russian Text © N.V. Karpova, T.S. Stytsenko, V.V. Yaderets, V.A. Andryushina, V.V. Dzhavakhiya, 2019, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2019, Vol. 55, No. 1, pp. 51–55. Optimization of the Method of Obtaining 9α-Hydroxy-androst-4-ene-3,17-dione—the Key Intermediate in the Synthesis of Highly Active Fluorinated Corticosteroids from Phytosterols a a a a, a N. V. Karpova , T. S. Stytsenko , V. V. Yaderets , V. A. Andryushina *, and V. V. Dzhavakhiya Bioengineering Institute, Biotechnology Research Center, Russian Academy of Sciences, Moscow, 119071 Russia *e-mail: andryushina@rambler.ru Received April 13, 2018; revised July 16, 2018; accepted July 25, 2018 Abstract—A method of phytosterol conversion into 9α-hydroxy-androstenedione under high loads of ste- roid substrate has been developed with the use of mixed cultures of the actinobacteria M. neoaurum and R. erythropolis. The introduction of a 9α-hydroxylating culture after the first 70 h of conversion made it possible to increase process selectivity and to exclude the use of methylcyclodextrin, which is expensive and diff icult to regenerate. Keywords: phytosterols, mixed cultures, 9α-hydroxylation, f luorinated corticosteroids DOI: 10.1134/S0003683819010071 INTRODUCTION 11-hydroxylation from the production process due to

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Applied Biochemistry and Microbiology – Springer Journals

Published: Apr 1, 2019

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