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Optimization of the Expression of Nitrilase from Alcaligenes denitrificans in Rhodococcus rhodochrous to Improve the Efficiency of Biocatalytic Synthesis of Ammonium Acrylate

Optimization of the Expression of Nitrilase from Alcaligenes denitrificans in Rhodococcus... ISSN 0003-6838, Applied Biochemistry and Microbiology, 2019, Vol. 55, No. 9, pp. 861–869. © Pleiades Publishing, Inc., 2019. Russian Text © The Author(s), 2019, published in Biotekhnologiya, 2019, Vol. 35, No. 1, pp. 27–37. PRODUCERS, BIOLOGY, SELECTION, AND GENE ENGINEERING Optimization of the Expression of Nitrilase from Alcaligenes denitrif icans in Rhodococcus rhodochrous to Improve the Eff iciency of Biocatalytic Synthesis of Ammonium Acrylate a, a a a a K. V. Lavrov *, E. G. Grechishnikova , A. O. Shemyakina , A. D. Novikov , T. I. Kalinina , b c c c a, A. S. Epremyan , S. A. Glinskii , R. A. Minasyan , S. P. Voronin , and A. S. Yanenko ** State Research Institute of Genetics and Selection of Industrial Microorganisms, Kurchatov Institute National Research Center (GosNIIgenetika, Kurchatov Institute NRC), Moscow, 117545 Russia Scientific Production Center Armbiotechnology, National Academy of Sciences of Armenia, Yerevan, 0056 Armenia BIOAMIDE Closed Joint Stock Venture, Saratov, 410033 Russia *e-mail: lavrov.ko@gmail.com **e-mail: yanenko@genetika.ru Received December 13, 2018; revised January 24, 2019; accepted February 15, 2019 Abstract—A Rhodococcus rhodochrous strain, M33-2nit, has been constructed with two copies of the nitrilase gene from A. denitrificans В-9582 under the control of nitrile hydratase http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Applied Biochemistry and Microbiology Springer Journals

Optimization of the Expression of Nitrilase from Alcaligenes denitrificans in Rhodococcus rhodochrous to Improve the Efficiency of Biocatalytic Synthesis of Ammonium Acrylate

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Publisher
Springer Journals
Copyright
Copyright © 2019 by Pleiades Publishing, Inc.
Subject
Life Sciences; Biochemistry, general; Microbiology; Medical Microbiology
ISSN
0003-6838
eISSN
1608-3024
DOI
10.1134/S0003683819090035
Publisher site
See Article on Publisher Site

Abstract

ISSN 0003-6838, Applied Biochemistry and Microbiology, 2019, Vol. 55, No. 9, pp. 861–869. © Pleiades Publishing, Inc., 2019. Russian Text © The Author(s), 2019, published in Biotekhnologiya, 2019, Vol. 35, No. 1, pp. 27–37. PRODUCERS, BIOLOGY, SELECTION, AND GENE ENGINEERING Optimization of the Expression of Nitrilase from Alcaligenes denitrif icans in Rhodococcus rhodochrous to Improve the Eff iciency of Biocatalytic Synthesis of Ammonium Acrylate a, a a a a K. V. Lavrov *, E. G. Grechishnikova , A. O. Shemyakina , A. D. Novikov , T. I. Kalinina , b c c c a, A. S. Epremyan , S. A. Glinskii , R. A. Minasyan , S. P. Voronin , and A. S. Yanenko ** State Research Institute of Genetics and Selection of Industrial Microorganisms, Kurchatov Institute National Research Center (GosNIIgenetika, Kurchatov Institute NRC), Moscow, 117545 Russia Scientific Production Center Armbiotechnology, National Academy of Sciences of Armenia, Yerevan, 0056 Armenia BIOAMIDE Closed Joint Stock Venture, Saratov, 410033 Russia *e-mail: lavrov.ko@gmail.com **e-mail: yanenko@genetika.ru Received December 13, 2018; revised January 24, 2019; accepted February 15, 2019 Abstract—A Rhodococcus rhodochrous strain, M33-2nit, has been constructed with two copies of the nitrilase gene from A. denitrificans В-9582 under the control of nitrile hydratase

Journal

Applied Biochemistry and MicrobiologySpringer Journals

Published: Dec 5, 2019

References