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R.M. Atlas (1993)
Handbook of Microbiology Media
A.K. Akatov, V.S. Zueva (1983)
Stafilokokki (Staphylococci)
The effect of culture conditions (nutrient media, aeration, and temperature) of Staphylococcus warneri KL-1 on the growth and production of the antibacterial peptide warnerin was studied. The selected medium allowed the intensive development of S. warnerin KL-1 culture and a high level of peptide accumulation. It was demonstrated that the bacterial culture growth rate and warnerin production depended on the extent of aeration of the nutrient medium. The highest antibacterial activity (ABA) was achieved in culture liquid by the fed-batch cultivation. The amino acid sequence of the warnerin pure form was determined by structural bioinformatics analysis, and the 3D structure of this peptide was constructed. It was found that the peptide molecule contained three thioether cycles formed by one lanthionine residue and two methyl-lanthionine residues. According to the current classification of lanthipeptides, warnerin can be attributed to class I of lantibiotics.
Applied Biochemistry and Microbiology – Springer Journals
Published: Feb 21, 2017
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