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O21 - The role of DNA damage and repair in allergic airway inflammation

O21 - The role of DNA damage and repair in allergic airway inflammation Chan et al. Clinical and Translational Allergy 2014, 4(Suppl 1):O21 http://www.ctajournal.com/content/4/S1/O21 ORAL PRESENTATION Open Access O21 - The role of DNA damage and repair in allergic airway inflammation 1,2,3* 1,2 1,2 3,4 1,2 Tze Khee Chan , Xin Yi Loh , Daniel WS Tan , Bevin P Engelward , Fred WS Wong From 3rd Pediatric Allergy and Asthma Meeting (PAAM) Athens, Greece. 17-19 October 2013 Introduction compare to no-drug treatment control. When DNA Extensive DNA damage and inefficient DNA repair repair was obstructed, apoptosis of airway epithelium might be responsible for some of the pathogenic fea- cells was enhanced. This indicates the important role of tures in patients suffering from asthma. To determine DNA repair in airway inflammation. whether DNA adducts can be used as a “dosimeter” for asthma disease severity, we measured the DNA adducts Acknowledgements level in lung of mouse with house dust mite (HDM)- This work was supported by BMRC grant 09/1/21/19/595 with additional induced allergic airway inflammation, as the disease pro- support from the Singapore-MIT Alliance for Research and Technology. gresses. Apoptosis of airway epithelial cells is one of the Authors’ details most critical pathophysiological factors in the develop- 1 Department of Pharmacology, Yong Loo Lin School of Medicine, National ment of chronic asthma. As repairing of DNA lesions is University Health System, Singapore. Immunology Program, Life Science Institute, National University of Singapore, Singapore. Interdisciplinary important in preventing apoptosis, we propose that Research Group in Infectious Diseases, Singapore-MIT Alliance for Research DNA repair plays an important pathophysiologic role in 4 and Technology (SMART), Singapore. Department of Biological Engineering, regulating lung epithelial cell DNA damage response. Massachusetts Institute of Technology, Cambridge, MA, USA. Published: 28 February 2014 Results We immunofluorescence-stained mice asthmatic lung tissue sections and observed an increase in DNA dou- doi:10.1186/2045-7022-4-S1-O21 ble strand break (DBS) markers, gH2AX and 53BP1 as Cite this article as: Chan et al.: O21 - The role of DNA damage and compared to control. Level of DNA repair proteins repair in allergic airway inflammation. Clinical and Translational Allergy 2014 4(Suppl 1):O21. that involved in homologous recombination and non- homologous end joining, were up-regulated substantially as early as 1 day-post last challenge. TUNEL assay revealed high level of DNA strand breaks in bronchial epithelium. DNA damage signaling pathway PCR array showed a reproducible increase in expression of multiple Submit your next manuscript to BioMed Central genes involved in DNA damage and repair. Treatment and take full advantage of: with glucocorticoid significantly reduced cell infiltration into airway as well as DNA damage and repair markers. • Convenient online submission To elucidate the role of DNA repair in regulating dis- • Thorough peer review ease outcome, we treated mice with NU7441, a DNA- • No space constraints or color figure charges dependent protein kinases inhibitor, and had observed • Immediate publication on acceptance an increased DNA damage makers expression in lung • Inclusion in PubMed, CAS, Scopus and Google Scholar and increased apoptotic level in bronchial epithelium as • Research which is freely available for redistribution Department of Pharmacology, Yong Loo Lin School of Medicine, National Submit your manuscript at University Health System, Singapore www.biomedcentral.com/submit Full list of author information is available at the end of the article © 2014 Tze et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Clinical and Translational Allergy Springer Journals

O21 - The role of DNA damage and repair in allergic airway inflammation

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Publisher
Springer Journals
Copyright
Copyright © 2014 by Tze et al; licensee BioMed Central Ltd.
Subject
Medicine & Public Health; Allergology; Immunology
eISSN
2045-7022
DOI
10.1186/2045-7022-4-S1-O21
Publisher site
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Abstract

Chan et al. Clinical and Translational Allergy 2014, 4(Suppl 1):O21 http://www.ctajournal.com/content/4/S1/O21 ORAL PRESENTATION Open Access O21 - The role of DNA damage and repair in allergic airway inflammation 1,2,3* 1,2 1,2 3,4 1,2 Tze Khee Chan , Xin Yi Loh , Daniel WS Tan , Bevin P Engelward , Fred WS Wong From 3rd Pediatric Allergy and Asthma Meeting (PAAM) Athens, Greece. 17-19 October 2013 Introduction compare to no-drug treatment control. When DNA Extensive DNA damage and inefficient DNA repair repair was obstructed, apoptosis of airway epithelium might be responsible for some of the pathogenic fea- cells was enhanced. This indicates the important role of tures in patients suffering from asthma. To determine DNA repair in airway inflammation. whether DNA adducts can be used as a “dosimeter” for asthma disease severity, we measured the DNA adducts Acknowledgements level in lung of mouse with house dust mite (HDM)- This work was supported by BMRC grant 09/1/21/19/595 with additional induced allergic airway inflammation, as the disease pro- support from the Singapore-MIT Alliance for Research and Technology. gresses. Apoptosis of airway epithelial cells is one of the Authors’ details most critical pathophysiological factors in the develop- 1 Department of Pharmacology, Yong Loo Lin School of Medicine, National ment of chronic asthma. As repairing of DNA lesions is University Health System, Singapore. Immunology Program, Life Science Institute, National University of Singapore, Singapore. Interdisciplinary important in preventing apoptosis, we propose that Research Group in Infectious Diseases, Singapore-MIT Alliance for Research DNA repair plays an important pathophysiologic role in 4 and Technology (SMART), Singapore. Department of Biological Engineering, regulating lung epithelial cell DNA damage response. Massachusetts Institute of Technology, Cambridge, MA, USA. Published: 28 February 2014 Results We immunofluorescence-stained mice asthmatic lung tissue sections and observed an increase in DNA dou- doi:10.1186/2045-7022-4-S1-O21 ble strand break (DBS) markers, gH2AX and 53BP1 as Cite this article as: Chan et al.: O21 - The role of DNA damage and compared to control. Level of DNA repair proteins repair in allergic airway inflammation. Clinical and Translational Allergy 2014 4(Suppl 1):O21. that involved in homologous recombination and non- homologous end joining, were up-regulated substantially as early as 1 day-post last challenge. TUNEL assay revealed high level of DNA strand breaks in bronchial epithelium. DNA damage signaling pathway PCR array showed a reproducible increase in expression of multiple Submit your next manuscript to BioMed Central genes involved in DNA damage and repair. Treatment and take full advantage of: with glucocorticoid significantly reduced cell infiltration into airway as well as DNA damage and repair markers. • Convenient online submission To elucidate the role of DNA repair in regulating dis- • Thorough peer review ease outcome, we treated mice with NU7441, a DNA- • No space constraints or color figure charges dependent protein kinases inhibitor, and had observed • Immediate publication on acceptance an increased DNA damage makers expression in lung • Inclusion in PubMed, CAS, Scopus and Google Scholar and increased apoptotic level in bronchial epithelium as • Research which is freely available for redistribution Department of Pharmacology, Yong Loo Lin School of Medicine, National Submit your manuscript at University Health System, Singapore www.biomedcentral.com/submit Full list of author information is available at the end of the article © 2014 Tze et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

Journal

Clinical and Translational Allergy – Springer Journals

Published: Apr 28, 2014

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