Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

New Insight Into Early Somatic Embryogenesis of Mangosteen (Garcinia mangostana) Through de Novo and Comparative Transcriptome Analyses

New Insight Into Early Somatic Embryogenesis of Mangosteen (Garcinia mangostana) Through de Novo... Somatic embryogenesis in mangosteen (Garcinia mangostana L.), an apomictic and recalcitrant-seeded species, allows stock improvement through genetic manipulation as well as mass propagation in a shortened time. To understand molecular events and gene expression during early somatic embryogenesis of mangosteen, we used RNA-Seq technology and assembled the mangosteen transcriptome de novo. Using the Trinity package, 186,203 transcripts were assembled with a mean size of 767 bp, 34.8 % of which showed significant similarities to known sequences in the GenBank non-redundant protein database. A total of 4001 transcripts were differentially expressed during somatic embryogenesis, while transcripts encoding signalling components of plant hormones such as auxin and cytokinin were significantly enriched. All the cytokinin-related genes were up-regulated, while some auxin-related genes were down-regulated and others were up-regulated. Somatic embryogenesis candidate genes such as SERK2, AGL15, CLVs, WUS and PIN1 were up-regulated in somatic embryos. Zinc finger and MADS family transcription factor genes were among the most presented differentially expressed transcripts in early somatic embryogenesis. Further qRT-PCR analysis confirmed the expression levels of the candidate genes. This study reports a de novo transcriptome assembly and analysis of gene expression during early somatic embryogenesis of mangosteen. Differentially expressed genes reveal that cytokinin has critical role in triggering somatic cell dedifferentiation and induction of somatic embryogenesis in mangosteen. The present work can serve as an important resource for further functional studies in woody plant embryogenesis. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Tropical Plant Biology Springer Journals

New Insight Into Early Somatic Embryogenesis of Mangosteen (Garcinia mangostana) Through de Novo and Comparative Transcriptome Analyses

Loading next page...
 
/lp/springer-journals/new-insight-into-early-somatic-embryogenesis-of-mangosteen-garcinia-Kt0qK1oxhU
Publisher
Springer Journals
Copyright
Copyright © 2016 by Springer Science+Business Media New York
Subject
Life Sciences; Plant Sciences; Plant Genetics & Genomics; Plant Breeding/Biotechnology; Plant Ecology; Transgenics
ISSN
1935-9756
eISSN
1935-9764
DOI
10.1007/s12042-016-9182-3
Publisher site
See Article on Publisher Site

Abstract

Somatic embryogenesis in mangosteen (Garcinia mangostana L.), an apomictic and recalcitrant-seeded species, allows stock improvement through genetic manipulation as well as mass propagation in a shortened time. To understand molecular events and gene expression during early somatic embryogenesis of mangosteen, we used RNA-Seq technology and assembled the mangosteen transcriptome de novo. Using the Trinity package, 186,203 transcripts were assembled with a mean size of 767 bp, 34.8 % of which showed significant similarities to known sequences in the GenBank non-redundant protein database. A total of 4001 transcripts were differentially expressed during somatic embryogenesis, while transcripts encoding signalling components of plant hormones such as auxin and cytokinin were significantly enriched. All the cytokinin-related genes were up-regulated, while some auxin-related genes were down-regulated and others were up-regulated. Somatic embryogenesis candidate genes such as SERK2, AGL15, CLVs, WUS and PIN1 were up-regulated in somatic embryos. Zinc finger and MADS family transcription factor genes were among the most presented differentially expressed transcripts in early somatic embryogenesis. Further qRT-PCR analysis confirmed the expression levels of the candidate genes. This study reports a de novo transcriptome assembly and analysis of gene expression during early somatic embryogenesis of mangosteen. Differentially expressed genes reveal that cytokinin has critical role in triggering somatic cell dedifferentiation and induction of somatic embryogenesis in mangosteen. The present work can serve as an important resource for further functional studies in woody plant embryogenesis.

Journal

Tropical Plant BiologySpringer Journals

Published: Sep 29, 2016

References