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New Associations of Aerobic Bacteria that Actively Decompose Lindane

New Associations of Aerobic Bacteria that Actively Decompose Lindane Associations of aerobic bacteria capable of decomposing the organochlorine pesticide lindane at a concentration of 0.1 g/L in a mineral medium for 30–180 days were obtained via selection. It was found that the lindane-degrading associations L2-6, L3-6, L4-6, L6-6, and L4-10 were characterized by a low level of species diversity (Shannon index of 1.88–2.46). The associations include representatives of the classes of γ‑Proteobacteria (the genus Pseudomonas) and α-Proteobacteria (the genera Novosphingobium, Sphingoauranticus, Sphingomonas, Tardibacter). It was shown that the total DNA isolated from the bacterial associations of the sixth passage (L2-6, L3-6, L4-6, and L6-6) contained genes with a 98–100% level of similarity with the linABCX genes, which encode the “upper” pathways for aerobic lindane transformation. The bacterial association L4-10 effectively decomposed lindane in a mineral medium (100% destruction of 0.1 and 0.3 g/kg of lindane for 30 and 90 days, respectively) and in model soil systems (78.1–90% destruction of 0.5 g/kg of lindane for 45 days). Molecular-genetic and analytical methods indicated that the L4-10 association decomposed not only lindane but also the byproduct chlorine-organic compounds that form during its metabolism (1,3,4-trichlorobenzene, 2,5-dichlorophenol). Thus, the bacterial associations obtained in this study are of interest for the development of bioremediation technologies for areas contaminated with lindane. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Applied Biochemistry and Microbiology Springer Journals

New Associations of Aerobic Bacteria that Actively Decompose Lindane

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Publisher
Springer Journals
Copyright
Copyright © Pleiades Publishing, Inc. 2021. ISSN 0003-6838, Applied Biochemistry and Microbiology, 2021, Vol. 57, No. 5, pp. 643–655. © Pleiades Publishing, Inc., 2021. Russian Text © The Author(s), 2021, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2021, Vol. 57, No. 5, pp. 504–518.
ISSN
0003-6838
eISSN
1608-3024
DOI
10.1134/s0003683821050112
Publisher site
See Article on Publisher Site

Abstract

Associations of aerobic bacteria capable of decomposing the organochlorine pesticide lindane at a concentration of 0.1 g/L in a mineral medium for 30–180 days were obtained via selection. It was found that the lindane-degrading associations L2-6, L3-6, L4-6, L6-6, and L4-10 were characterized by a low level of species diversity (Shannon index of 1.88–2.46). The associations include representatives of the classes of γ‑Proteobacteria (the genus Pseudomonas) and α-Proteobacteria (the genera Novosphingobium, Sphingoauranticus, Sphingomonas, Tardibacter). It was shown that the total DNA isolated from the bacterial associations of the sixth passage (L2-6, L3-6, L4-6, and L6-6) contained genes with a 98–100% level of similarity with the linABCX genes, which encode the “upper” pathways for aerobic lindane transformation. The bacterial association L4-10 effectively decomposed lindane in a mineral medium (100% destruction of 0.1 and 0.3 g/kg of lindane for 30 and 90 days, respectively) and in model soil systems (78.1–90% destruction of 0.5 g/kg of lindane for 45 days). Molecular-genetic and analytical methods indicated that the L4-10 association decomposed not only lindane but also the byproduct chlorine-organic compounds that form during its metabolism (1,3,4-trichlorobenzene, 2,5-dichlorophenol). Thus, the bacterial associations obtained in this study are of interest for the development of bioremediation technologies for areas contaminated with lindane.

Journal

Applied Biochemistry and MicrobiologySpringer Journals

Published: Sep 1, 2021

Keywords: bacterial associations; lindane; destruction; lin genes

References