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Morphological and molecular confirmation of Xiphinema brevicollum, a nematode from the virus-vectoring X. americanum group, from New Zealand

Morphological and molecular confirmation of Xiphinema brevicollum, a nematode from the... Some nematode species in the Longidoridae are important agricultural pests because they are vectors of nepoviruses. Species in the Xiphinema americanum group have been widely studied because they transmit at least four nepoviruses. The identity, taxonomy and worldwide distribution of nematodes in the group remain much debated. At least three species in this group have been described from New Zealand, but DNA techniques were not used in these studies. These techniques are highly discriminatory for identifying closely related species. We are in the process of barcoding New Zealand nematodes for biosecurity and pest management purposes. Here we describe the identification of X. brevicollum, a nematode from the X. americanum group, using DNA sequencing of the ribosomal large subunit D2/D3 and internal transcribed spacer-1 regions, from New Zealand. Additional keywords: cloning, ZyGEM DNA extraction. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Australasian Plant Pathology Springer Journals

Morphological and molecular confirmation of Xiphinema brevicollum, a nematode from the virus-vectoring X. americanum group, from New Zealand

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References (38)

Publisher
Springer Journals
Copyright
Copyright © 2009 by Australasian Plant Pathology Society
Subject
Life Sciences; Plant Pathology; Plant Sciences; Agriculture; Entomology; Ecology
ISSN
0815-3191
eISSN
1448-6032
DOI
10.1071/AP09034
Publisher site
See Article on Publisher Site

Abstract

Some nematode species in the Longidoridae are important agricultural pests because they are vectors of nepoviruses. Species in the Xiphinema americanum group have been widely studied because they transmit at least four nepoviruses. The identity, taxonomy and worldwide distribution of nematodes in the group remain much debated. At least three species in this group have been described from New Zealand, but DNA techniques were not used in these studies. These techniques are highly discriminatory for identifying closely related species. We are in the process of barcoding New Zealand nematodes for biosecurity and pest management purposes. Here we describe the identification of X. brevicollum, a nematode from the X. americanum group, using DNA sequencing of the ribosomal large subunit D2/D3 and internal transcribed spacer-1 regions, from New Zealand. Additional keywords: cloning, ZyGEM DNA extraction.

Journal

Australasian Plant PathologySpringer Journals

Published: Jan 18, 2011

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