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Molecular genetic features of 5S rDNA nontranscribed spacer in Hippophae rhamnoides L.

Molecular genetic features of 5S rDNA nontranscribed spacer in Hippophae rhamnoides L. Amplification of sea buckthorn Hippophae rhamnoides L. 5S rDNA nontranscribed spacer with coding border anneal primers showed existence of a single fragment. The fragment was cloned and sequenced. It was shown that length of the Hippophae rhamnoides L. 5S rDNA nontranscribed spacer is 807 bp. Analysis of the sequence allowed to detect a high homology with early described microsatellite locuses of Hippophae rhamnoides L., russian olive Elaeagnus angustifolia L., and Calligonum mongolicum Turcz. that include a (GA)9 motif. These results may be useful to study a ribosomal RNA gene organization. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Moscow University Biological Sciences Bulletin Springer Journals

Molecular genetic features of 5S rDNA nontranscribed spacer in Hippophae rhamnoides L.

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References (23)

Publisher
Springer Journals
Copyright
Copyright © 2016 by Allerton Press, Inc.
Subject
Life Sciences; Biochemistry, general; Cell Biology; Life Sciences, general; Plant Sciences; Zoology
ISSN
0096-3925
eISSN
1934-791X
DOI
10.3103/S0096392516040015
Publisher site
See Article on Publisher Site

Abstract

Amplification of sea buckthorn Hippophae rhamnoides L. 5S rDNA nontranscribed spacer with coding border anneal primers showed existence of a single fragment. The fragment was cloned and sequenced. It was shown that length of the Hippophae rhamnoides L. 5S rDNA nontranscribed spacer is 807 bp. Analysis of the sequence allowed to detect a high homology with early described microsatellite locuses of Hippophae rhamnoides L., russian olive Elaeagnus angustifolia L., and Calligonum mongolicum Turcz. that include a (GA)9 motif. These results may be useful to study a ribosomal RNA gene organization.

Journal

Moscow University Biological Sciences BulletinSpringer Journals

Published: Dec 28, 2016

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