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Molecular and seroprevalence of imported dengue virus infection in Al-Madinah, Saudi Arabia

Molecular and seroprevalence of imported dengue virus infection in Al-Madinah, Saudi Arabia Recently, urban outbreaks of dengue virus (DENV) have occurred in western areas of Saudi Arabia; Jeddah and Makkah, neighbouring cities to Al-Madinah, where there is growing population traffic. DENV activity has not previously been reported in Al-Madinah. Molecular detection of DENV RNA using type-specific single-step real-time RT-PCR and seroprevalence of anti-DENV antibodies using ELISA was reported among Al-Madinah population for the first time through a cross-sectional study from May 2008 to July 2009. A total of 351 febrile, clinically suspected patients were identified, and acute dengue infection was identified in 71 of them during the first week of onset of fever; 5 (1.4 %) by real-time reverse transcription PCR alone, 45 (12.8 %) cases by IgM-ELISA alone and 13 (3.7 %) by both, while 8 (2.3 %) cases were identified during the second week of fever by the presence of IgM-ELISA only. Anti-DENV IgG antibodies were not detected in any of the tested samples. Of the 71 cases, 55 were resident in Al-Madinah (37 Saudi and 18 non-Saudi); however, all of them were imported cases. DENV-1 and DENV-2 were identified in 7 and 11 cases, respectively, while DENV-3 and DENV-4 were not detected in any cases. It was observed that the middle-aged group was the most infected group. DENV anti-DENV IgM antibodies showed a positive correlation of high significance with the number of days with fever. Nationality and gender were found to be significant independent predictors. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Comparative Clinical Pathology Springer Journals

Molecular and seroprevalence of imported dengue virus infection in Al-Madinah, Saudi Arabia

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References (53)

Publisher
Springer Journals
Copyright
Copyright © 2013 by Springer-Verlag London
Subject
Medicine & Public Health; Pathology; Hematology; Oncology
eISSN
1618-565X
DOI
10.1007/s00580-013-1704-x
Publisher site
See Article on Publisher Site

Abstract

Recently, urban outbreaks of dengue virus (DENV) have occurred in western areas of Saudi Arabia; Jeddah and Makkah, neighbouring cities to Al-Madinah, where there is growing population traffic. DENV activity has not previously been reported in Al-Madinah. Molecular detection of DENV RNA using type-specific single-step real-time RT-PCR and seroprevalence of anti-DENV antibodies using ELISA was reported among Al-Madinah population for the first time through a cross-sectional study from May 2008 to July 2009. A total of 351 febrile, clinically suspected patients were identified, and acute dengue infection was identified in 71 of them during the first week of onset of fever; 5 (1.4 %) by real-time reverse transcription PCR alone, 45 (12.8 %) cases by IgM-ELISA alone and 13 (3.7 %) by both, while 8 (2.3 %) cases were identified during the second week of fever by the presence of IgM-ELISA only. Anti-DENV IgG antibodies were not detected in any of the tested samples. Of the 71 cases, 55 were resident in Al-Madinah (37 Saudi and 18 non-Saudi); however, all of them were imported cases. DENV-1 and DENV-2 were identified in 7 and 11 cases, respectively, while DENV-3 and DENV-4 were not detected in any cases. It was observed that the middle-aged group was the most infected group. DENV anti-DENV IgM antibodies showed a positive correlation of high significance with the number of days with fever. Nationality and gender were found to be significant independent predictors.

Journal

Comparative Clinical PathologySpringer Journals

Published: Feb 24, 2013

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