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Modulation of mouse neutrophil cytokine secretion by Klebsiella pneumoniae

Modulation of mouse neutrophil cytokine secretion by Klebsiella pneumoniae Previous studies have shown that Klebsiella pneumoniae (Kle.p) is an immune function modulator for both neutrophils (polymorphonuclear leukocytes, PMN)s and mononuclear cells. To investigate further the effects of Kle.p on the expression of various cytokines secreted by neutrophils and the subsequent modulation pathways, we measured the expression of tumour necrosis factor (TNF)-α, interleukin (IL)-1β and IκB by reverse transcriptase-polymerase chain reaction (RT-PCR), enzyme-linked immunosorbent assay (ELISA), gene micro-array or Western blot before and after Kle.p infection. Neutrophils were shown to express many cytokines and chemokines, including TNF-α, IL-1α, IL-1β, IL-2, nuclear factor (NF)κB, INF-α and INF-β, etc. Kle.p treatment increased neutrophil cytokine production, and this was correlated to a reduction of IκB expression of neutrophils. It was concluded that Kle.p infection can upregulate the immunomodulating function of neutrophils by secreting more cytokines, possibly through the NFκB–IκB pathway. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Comparative Clinical Pathology Springer Journals

Modulation of mouse neutrophil cytokine secretion by Klebsiella pneumoniae

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References (23)

Publisher
Springer Journals
Copyright
Copyright © 2004 by Springer-Verlag London Limited
Subject
Medicine & Public Health; Pathology; Hematology; Oncology
eISSN
1618-565X
DOI
10.1007/s00580-004-0512-8
Publisher site
See Article on Publisher Site

Abstract

Previous studies have shown that Klebsiella pneumoniae (Kle.p) is an immune function modulator for both neutrophils (polymorphonuclear leukocytes, PMN)s and mononuclear cells. To investigate further the effects of Kle.p on the expression of various cytokines secreted by neutrophils and the subsequent modulation pathways, we measured the expression of tumour necrosis factor (TNF)-α, interleukin (IL)-1β and IκB by reverse transcriptase-polymerase chain reaction (RT-PCR), enzyme-linked immunosorbent assay (ELISA), gene micro-array or Western blot before and after Kle.p infection. Neutrophils were shown to express many cytokines and chemokines, including TNF-α, IL-1α, IL-1β, IL-2, nuclear factor (NF)κB, INF-α and INF-β, etc. Kle.p treatment increased neutrophil cytokine production, and this was correlated to a reduction of IκB expression of neutrophils. It was concluded that Kle.p infection can upregulate the immunomodulating function of neutrophils by secreting more cytokines, possibly through the NFκB–IκB pathway.

Journal

Comparative Clinical PathologySpringer Journals

Published: Jun 4, 2004

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