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Modification of a System Based on the Use of Selection and Sorting Markers for the Screening of Stable Transfectants

Modification of a System Based on the Use of Selection and Sorting Markers for the Screening of... ISSN 0003-6838, Applied Biochemistry and Microbiology, 2018, Vol. 54, No. 9, pp. 842–848. © Pleiades Publishing, Inc., 2018. Original Russian Text © I.G. Vorobyova, R.R. Shukurov, D.G. Kozlov, T.B. Koryagina, N.V. Antipova, V.N. Stepanenko, 2018, published in Biotekhnologiya, 2018, Vol. 34, No. 2, pp. 9–17. PRODUCING ORGANISMS, BIOLOGY, SELECTION, AND GENETIC ENGINEERING Modif ication of a System Based on the Use of Selection and Sorting Markers for the Screening of Stable Transfectants a, b c I. G. Vorobyova *, R. R. Shukurov , D. G. Kozlov , a a a T. B. Koryagina , N. V. Antipova , and V. N. Stepanenko Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, 117997 Russia LLC International Biotechnology Center Generium, Moscow, 127006 Russia State Research Institute for Genetics and Selection of Industrial Microorganisms, Kurchatov Institute National Research Center, Moscow, 117545 Russia *e-mail: iva1647@yandex.ru Received December 4, 2017; in final form, March 1, 2018 Abstract⎯ The р-hook technique, which is based on protein domain expression on the cell surface, has been modified and used for the selection and highly sensitive and efficient sorting of stably transfected CHO-S cells. The model protein EYFP served as both the target protein produced http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Applied Biochemistry and Microbiology Springer Journals

Modification of a System Based on the Use of Selection and Sorting Markers for the Screening of Stable Transfectants

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References (19)

Publisher
Springer Journals
Copyright
Copyright © 2018 by Pleiades Publishing, Inc.
Subject
Life Sciences; Biochemistry, general; Microbiology; Medical Microbiology
ISSN
0003-6838
eISSN
1608-3024
DOI
10.1134/S0003683818090077
Publisher site
See Article on Publisher Site

Abstract

ISSN 0003-6838, Applied Biochemistry and Microbiology, 2018, Vol. 54, No. 9, pp. 842–848. © Pleiades Publishing, Inc., 2018. Original Russian Text © I.G. Vorobyova, R.R. Shukurov, D.G. Kozlov, T.B. Koryagina, N.V. Antipova, V.N. Stepanenko, 2018, published in Biotekhnologiya, 2018, Vol. 34, No. 2, pp. 9–17. PRODUCING ORGANISMS, BIOLOGY, SELECTION, AND GENETIC ENGINEERING Modif ication of a System Based on the Use of Selection and Sorting Markers for the Screening of Stable Transfectants a, b c I. G. Vorobyova *, R. R. Shukurov , D. G. Kozlov , a a a T. B. Koryagina , N. V. Antipova , and V. N. Stepanenko Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, 117997 Russia LLC International Biotechnology Center Generium, Moscow, 127006 Russia State Research Institute for Genetics and Selection of Industrial Microorganisms, Kurchatov Institute National Research Center, Moscow, 117545 Russia *e-mail: iva1647@yandex.ru Received December 4, 2017; in final form, March 1, 2018 Abstract⎯ The р-hook technique, which is based on protein domain expression on the cell surface, has been modified and used for the selection and highly sensitive and efficient sorting of stably transfected CHO-S cells. The model protein EYFP served as both the target protein produced

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Applied Biochemistry and Microbiology – Springer Journals

Published: Feb 26, 2019

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