Access the full text.
Sign up today, get DeepDyve free for 14 days.
Loading next page...
/lp/springer-journals/microarray-of-stimuli-responsive-microbeads-for-duplexed-immunoassay-G6ctNKG6yM
References (19)
-
S. Grego, Thomas Jarvis, B. Stoner, Jay Lewis (2005)
Template-directed assembly on an ordered microsphere array.Langmuir : the ACS journal of surfaces and colloids, 21 11
-
H. Härmä, P. Tarkkinen, T. Soukka, T. Lövgren (2000)
Miniature single-particle immunoassay for prostate-specific antigen in serum using recombinant Fab fragments.Clinical chemistry, 46 11
-
J. Goddard, S. Mandal, S. Nugen, A. Baeumner, D. Erickson (2010)
Biopatterning for label-free detection.Colloids and surfaces. B, Biointerfaces, 76 1
-
C. Roe, P. Courtoy, P. Baudhuin (1987)
A model of protein-colloidal gold interactions.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 35
-
M. Nichkova, D. Dosev, S. Gee, B. Hammock, I. Kennedy (2007)
Multiplexed immunoassays for proteins using magnetic luminescent nanoparticles for internal calibration.Analytical biochemistry, 369 1
-
D. Urry (1997)
Physical Chemistry of Biological Free Energy Transduction As Demonstrated by Elastic Protein-Based Polymers†Journal of Physical Chemistry B, 101
-
M. Alonso, V. Reboto, L. Guiscardo, V. Mate, J. Rodríguez‐Cabello (2001)
Novel Photoresponsive p-Phenylazobenzene Derivative of an Elastin-like Polymer with Enhanced Control of Azobenzene Content and without pH SensitivenessMacromolecules, 34
-
P. Montagne, P. Varcin, M. Cuillière, J. Duheille (1992)
Microparticle-enhanced nephelometric immunoassay with microsphere-antigen conjugates.Bioconjugate chemistry, 3 2
-
L. Lucas, Jin-Hee Han, Jeong‐Yeol Yoon (2006)
Using highly carboxylated microspheres to simplify immunoassays and enhance diffusional mixing in a microfluidic device.Colloids and surfaces. B, Biointerfaces, 49 2
-
Jingwen Chen, Marie Iannone, May-Sung Li, J. Taylor, Philip Rivers, Anita Nelsen, Kimberly Slentz-Kesler, Allen Roses, Michael Weiner (2000)
A microsphere-based assay for multiplexed single nucleotide polymorphism analysis using single base chain extension.Genome research, 10 4
-
Mrinmoy De, P. Ghosh, V. Rotello (2008)
Applications of Nanoparticles in BiologyAdvanced Materials, 20
-
Xiaomei Yan, Erika Schielke, K. Grace, C. Hassell, B. Marrone, J. Nolan (2004)
Microsphere-based duplexed immunoassay for influenza virus typing by flow cytometry.Journal of immunological methods, 284 1-2
-
Marie Iannone, Thomas Consler, Kenneth Pearce, J. Stimmel, Derek Parks, John Gray (2001)
Multiplexed molecular interactions of nuclear receptors using fluorescent microspheres.Cytometry, 44 4
-
I. Hsing, Ying Xu, Wenting Zhao (2007)
Micro‐ and Nano‐ Magnetic Particles for Applications in BiosensingElectroanalysis, 19
-
J. Ng, E. Selamat, Wen-Tso Liu (2008)
A spatially addressable bead-based biosensor for simple and rapid DNA detection.Biosensors & bioelectronics, 23 6
-
A. Girotti, J. Reguera, F. Arias, M. Alonso, A. Testera, J. Rodríguez‐Cabello (2004)
Influence of the Molecular Weight on the Inverse Temperature Transition of a Model Genetically Engineered Elastin-like pH-Responsive PolymerMacromolecules, 37
-
D. Peyrade, M. Gordon, G. Hyvert, K. Berton, J. Tallal (2006)
Direct observation and localization of colloidal nanoparticles on patterned surface by capillary forcesMicroelectronic Engineering, 83
-
D. Holmes, Joseph She, P. Roach, H. Morgan (2007)
Bead-based immunoassays using a micro-chip flow cytometer.Lab on a chip, 7 8
-
D. Vignali (2000)
Multiplexed particle-based flow cytometric assays.Journal of immunological methods, 243 1-2
- Publisher
- Springer Journals
- Copyright
- 2011 The Korean BioChip Society and Springer-Verlag Berlin Heidelberg
- ISSN
- 1976-0280
- eISSN
- 2092-7843
- DOI
- 10.1007/s13206-011-5209-x
- Publisher site
- See Article on Publisher Site
Abstract
Abstract This paper describes a duplexed immunoassay method based on a functional microbeads (μbeads) array. For selective detection of dual analytes, the μbeads were conjugated with a responsive biopolymer, characterized by a different phase transition temperature. Then, the μbeads modified with elastin-like polypeptides (ELPs) were spatially localized in a single microwell through sequential injection with a buffer. Prostate specific antigen (PSA) and mouse immunoglobulin G (IgG) were selected to show the feasibility of duplexed immunoassay. The detection limit of the μbead array with PSA and mouse IgG was 0.05 ng/mL and 0.1 ng/mL, respectively.
Journal
BioChip Journal – Springer Journals
Published: Jun 1, 2011