Access the full text.
Sign up today, get DeepDyve free for 14 days.
K. Mitchell, M. Weiss, B. Mitchell, P. Martin, D. Davis, L. Morales, B. Helwig, Mark Beerenstrauch, K. Abou-Easa, Tammi Hildreth, D. Troyer (2003)
Matrix Cells from Wharton's Jelly Form Neurons and GliaSTEM CELLS, 21
V. Paunescu, E. Deak, Diana Herman, I. Siska, Gabriela T˘anasie, C. Bunu, S. Anghel, C. Tatu, Tudor Oprea, R. Henschler, B. Rüster, Roxana Bistrian, E. Seifried (2007)
In vitro differentiation of human mesenchymal stem cells to epithelial lineageJournal of Cellular and Molecular Medicine, 11
R. Sarugaser, D. Lickorish, D. Baksh, M. Hosseini, J. Davies (2005)
Human Umbilical Cord Perivascular (HUCPV) Cells: A Source of Mesenchymal ProgenitorsSTEM CELLS, 23
A. Al-khaldi, H. Al-Sabti, J. Galipeau, K. Lachapelle (2003)
Therapeutic angiogenesis using autologous bone marrow stromal cells: improved blood flow in a chronic limb ischemia model.The Annals of thoracic surgery, 75 1
D. Troyer, M. Weiss (2008)
Concise Review: Wharton's Jelly‐Derived Cells Are a Primitive Stromal Cell PopulationSTEM CELLS, 26
N. Nardi, L. Meirelles (2006)
Mesenchymal stem cells: isolation, in vitro expansion and characterization.Handbook of experimental pharmacology
M. Sasaki, R. Abe, Y. Fujita, S. Ando, D. Inokuma, H. Shimizu (2008)
Mesenchymal Stem Cells Are Recruited into Wounded Skin and Contribute to Wound Repair by Transdifferentiation into Multiple Skin Cell Type1The Journal of Immunology, 180
S. Chin, Alfred Poey, C. Wong, Sau-Kong Chang, William Teh, T. Mohr, S. Cheong (2010)
Cryopreserved mesenchymal stromal cell treatment is safe and feasible for severe dilated ischemic cardiomyopathy.Cytotherapy, 12 1
Hwai-Shi Wang, S. Hung, Shu-Tine Peng, Chun-Chieh Huang, Hung-Mu Wei, Yi‐Jhih Guo, Yu-Show Fu, Mei-Chun Lai, Chin-Chang Chen (2004)
Mesenchymal Stem Cells in the Wharton's Jelly of the Human Umbilical CordSTEM CELLS, 22
D. Covas, J. Siufi, A. Silva, M. Orellana (2003)
Isolation and culture of umbilical vein mesenchymal stem cells.Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas, 36 9
H. Wang, S. Hung (2004)
MESENCHYMAL STEM CELLS IN THE WHARTONS JELLY OF THE HUMAN UMBILICAL CORD, 22
M. Weiss, S. Medicetty, A. Bledsoe, R. Rachakatla, Michael Choi, S. Merchav, Yongquan Luo, M. Rao, G. Velagaleti, D. Troyer (2006)
Human Umbilical Cord Matrix Stem Cells: Preliminary Characterization and Effect of Transplantation in a Rodent Model of Parkinson's DiseaseSTEM CELLS, 24
C. Fong, Li-Ling Chak, A. Biswas, J. Tan, K. Gauthaman, W. Chan, A. Bongso (2011)
Human Wharton’s Jelly Stem Cells Have Unique Transcriptome Profiles Compared to Human Embryonic Stem Cells and Other Mesenchymal Stem CellsStem Cell Reviews and Reports, 7
M. Weiss, Cameron Anderson, S. Medicetty, Kiran Seshareddy, R. Weiss, I. VanderWerff, D. Troyer, K. Mcintosh (2008)
Immune Properties of Human Umbilical Cord Wharton's Jelly‐Derived CellsSTEM CELLS, 26
Mengchao Wu, Ling Yang, Shu-qin Liu, Hengyu Li, N. Hui, Fubo Wang, Hou-qi Liu (2006)
Differentiation potential of human embryonic mesenchymal stem cells for skin‐related tissueBritish Journal of Dermatology, 155
A. Marmotti, S. Mattia, M. Bruzzone, Stefano Buttiglieri, Alessandra Risso, D. Bonasia, D. Blonna, F. Castoldi, Roberto Rossi, Cristina Zanini, Elisabetta Ercole, E. Defabiani, Corrado Tarella, Giuseppe Peretti (2012)
Minced Umbilical Cord Fragments as a Source of Cells for Orthopaedic Tissue Engineering: An In Vitro StudyStem Cells International, 2012
C. Capelli, E. Gotti, M. Morigi, Cinzia Rota, L. Weng, F. Dazzi, O. Spinelli, G. Cazzaniga, Rosangela Trezzi, A. Gianatti, A. Rambaldi, J. Golay, M. Introna (2011)
Minimally manipulated whole human umbilical cord is a rich source of clinical-grade human mesenchymal stromal cells expanded in human platelet lysate.Cytotherapy, 13 7
Yaojiong Wu, Liwen Chen, P. Scott, E. Tredget (2007)
Mesenchymal Stem Cells Enhance Wound Healing Through Differentiation and AngiogenesisSTEM CELLS, 25
J. Dennis, A. Merriam, Amad Awadallah, Jung Yoo, B. Johnstone, A. Caplan (1999)
A Quadripotential Mesenchymal Progenitor Cell Isolated from the Marrow of an Adult MouseJournal of Bone and Mineral Research, 14
P. Montanucci, G. Basta, T. Pescara, I. Pennoni, Francesca Giovanni, R. Calafiore (2011)
New simple and rapid method for purification of mesenchymal stem cells from the human umbilical cord Wharton jelly.Tissue engineering. Part A, 17 21-22
Mesenchymal stem cells (MSCs) are a group of cells present in bone marrow stroma, cord blood, Wharton’s jelly, and the stroma of various organs with the capacity for mesoderm-like cell differentiation. The aim of the present work is to isolate MSCs from human umbilical cord Wharton’s jelly and to examine the differentiation potential of these (MSCs) toward the epithelial lineage. Human MSCs derived from Wharton’s jelly of umbilical cord (≈31 cm) after a full-term delivery of 10 cases were localized and isolated and further cultured to enrich and enhance their proliferation. These MSCs were detected after an average of 21–30 days not only morphologically, as a uniform spindle fibroblast-like cells, and reached 70–80 % confluence with a good cellular yield, but also via their immunophenotypic analysis which showed positivity for CD29, CD90, CD105, and CD166 and negativity for CD34. All these cells showed 100 % negativity with CK19 to ensure the absence of any epithelial cells. Later, their differentiation potential was determined by culturing with conditioning medium that include bone morphogenetic protein-4, ascorbic acid, and human epithelium growth factor in a complete medium. These sets of conditions resulted in the expression of keratinocyte markers, namely pancytokeratin (mean value 92 %) and cytokeratin19 (mean value 74 %) using immunohistochemistry. In conclusion, these findings may have a significant impact on studies of early human epithelial differentiation, functional genomics, pharmacological testing, cell therapy, and tissue engineering by helping to eliminate worrying ethical and technical issues.
Comparative Clinical Pathology – Springer Journals
Published: Feb 24, 2013
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.