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LTP cross-reactivity - primary sensitization to mugwort pollen LTP Art v 3, facilitates subsequent sensitisation to peach LTP Pru p 3 in mice

LTP cross-reactivity - primary sensitization to mugwort pollen LTP Art v 3, facilitates... Wangorsch et al. Clinical and Translational Allergy 2014, 4(Suppl 2):O14 http://www.ctajournal.com/content/4/S2/O14 ORAL PRESENTATION Open Access LTP cross-reactivity - primary sensitization to mugwort pollen LTP Art v 3, facilitates subsequent sensitisation to peach LTP Pru p 3 in mice 1* 1 2 1 2 Andrea Wangorsch , Stefan Schülke , Gabriele Gadermaier , Melanie Albrecht , Michael Wallner , 1 1 3 1 2 4,4 1 Stefanie Randow , Sonja Wolfheimer , Jonas Lidholm , Iris Lauer , Fatima Ferreira , Masako Toda , Stefan Vieths , 1 1 Gerald Reese , Stephan Scheurer From 5th International Symposium on Molecular Allergology (ISMA 2013) Vienna, Austria. 6-7 December 2013 Non-specific lipid transfer proteins (nsLTPs) are impor- different antigenicity. In comparison to Art v 3, Pru p 3 tant pollen and food allergens in the Mediterranean area. showed much lower IgE-sensitizing capacity. In contrast, IgE response to Pru p 3 was promoted in mice previously Peach LTP Pru p 3 is considered as primary sensitizer inducing LTP cross-reactive IgE antibodies. Despite the sensitized to Art v 3, which was even the case upon con- evidence on the importance of Pru p 3, it is unclear current administration of both LTPs. Neither sequential whether reactivity to multiple LTPs is due to antibody immunization nor immunization with the mixture induced cross-reactivity or to independent sensitization events. IgE antibodies to unrelated LTPs, e.g. Cor a 8. In summary, Aim of the study was (1) to compare antigenicity of LTPs theimmuneresponsetoLTPsinmicevariedstrongly from pollen and food, and (2) to investigate whether between strains. The LTPs studied, displayed different primary sensitization to LTPs promotes secondary sensiti- immunogenic properties and did not induce cross-reactive zation to LTPs from unrelated sources. IgE sensitization to IgE antibodies. Primary sensitization to the pollen LTP Art peach LTP was compared in different mouse strains v 3 conditioned the animals for subsequent sensitization to (C3H/HeJ, BALB/c, CBA/J). IgE high responder mice the food LTP Pru p 3. The extent to which the findings are (CBA/J) were used to compare the antigenicity and IgE applicable to the manifestation of clinical cross-reactivity cross-reactivity of LTPs from pollen (mugwort/Art v 3, to LTPs in humans needs to be investigated. plane tree/Pla a 3, ragweed/Amb a 6) and foods (peach/ Pru p 3, cherry/Pru av 3, hazelnut/Cor a 8). To investigate Authors’ details whether an established Th2 immune response to LTPs 1 2 Paul-Ehrlich-Institut, Division of Allergology, Langen, Germany. University of facilitates a secondary immune response and cross-reactiv- Salzburg, Department of Molecular Biology, Salzburg, Austria. Thermo Fisher Scientific, Research and Development, Uppsala, Sweden. Paul-Ehrlich-Institut, ity to unrelated LTPs, CBA/J mice were immunized with Junior Research Group 1 Experimental Allergology, Langen, Germany. (1) Pru p 3 followed by Art v 3, (2) Art v 3 followed by Pru p 3, and (3) a mixture of Pru p 3 and Art v 3. Induc- Published: 17 March 2014 tion of Pru p 3 and Art v 3 specific IgE and IgG titers, and cross-reactivity between the two LTPs were determined by ELISA. CBA and C3H mice showed strong IgE-responses doi:10.1186/2045-7022-4-S2-O14 Cite this article as: Wangorsch et al.: LTP cross-reactivity - primary to Pru p 3, whereas BALB/c mice did not. Remarkably, a sensitization to mugwort pollen LTP Art v 3, facilitates subsequent species-specific immune response lacking any IgE- and sensitisation to peach LTP Pru p 3 in mice. Clinical and Translational Allergy IgG-cross-reactivity was observed for all tested LTPs 2014 4(Suppl 2):O14. except for Pru p 3 and Pru av 3. Although LTPs show high structural similarity, Prup3andArt v3displayed Paul-Ehrlich-Institut, Division of Allergology, Langen, Germany Full list of author information is available at the end of the article © 2014 Wangorsch et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Clinical and Translational Allergy Springer Journals

LTP cross-reactivity - primary sensitization to mugwort pollen LTP Art v 3, facilitates subsequent sensitisation to peach LTP Pru p 3 in mice

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Publisher
Springer Journals
Copyright
Copyright © 2014 by Wangorsch et al; licensee BioMed Central Ltd.
Subject
Medicine & Public Health; Allergology; Immunology
eISSN
2045-7022
DOI
10.1186/2045-7022-4-S2-O14
Publisher site
See Article on Publisher Site

Abstract

Wangorsch et al. Clinical and Translational Allergy 2014, 4(Suppl 2):O14 http://www.ctajournal.com/content/4/S2/O14 ORAL PRESENTATION Open Access LTP cross-reactivity - primary sensitization to mugwort pollen LTP Art v 3, facilitates subsequent sensitisation to peach LTP Pru p 3 in mice 1* 1 2 1 2 Andrea Wangorsch , Stefan Schülke , Gabriele Gadermaier , Melanie Albrecht , Michael Wallner , 1 1 3 1 2 4,4 1 Stefanie Randow , Sonja Wolfheimer , Jonas Lidholm , Iris Lauer , Fatima Ferreira , Masako Toda , Stefan Vieths , 1 1 Gerald Reese , Stephan Scheurer From 5th International Symposium on Molecular Allergology (ISMA 2013) Vienna, Austria. 6-7 December 2013 Non-specific lipid transfer proteins (nsLTPs) are impor- different antigenicity. In comparison to Art v 3, Pru p 3 tant pollen and food allergens in the Mediterranean area. showed much lower IgE-sensitizing capacity. In contrast, IgE response to Pru p 3 was promoted in mice previously Peach LTP Pru p 3 is considered as primary sensitizer inducing LTP cross-reactive IgE antibodies. Despite the sensitized to Art v 3, which was even the case upon con- evidence on the importance of Pru p 3, it is unclear current administration of both LTPs. Neither sequential whether reactivity to multiple LTPs is due to antibody immunization nor immunization with the mixture induced cross-reactivity or to independent sensitization events. IgE antibodies to unrelated LTPs, e.g. Cor a 8. In summary, Aim of the study was (1) to compare antigenicity of LTPs theimmuneresponsetoLTPsinmicevariedstrongly from pollen and food, and (2) to investigate whether between strains. The LTPs studied, displayed different primary sensitization to LTPs promotes secondary sensiti- immunogenic properties and did not induce cross-reactive zation to LTPs from unrelated sources. IgE sensitization to IgE antibodies. Primary sensitization to the pollen LTP Art peach LTP was compared in different mouse strains v 3 conditioned the animals for subsequent sensitization to (C3H/HeJ, BALB/c, CBA/J). IgE high responder mice the food LTP Pru p 3. The extent to which the findings are (CBA/J) were used to compare the antigenicity and IgE applicable to the manifestation of clinical cross-reactivity cross-reactivity of LTPs from pollen (mugwort/Art v 3, to LTPs in humans needs to be investigated. plane tree/Pla a 3, ragweed/Amb a 6) and foods (peach/ Pru p 3, cherry/Pru av 3, hazelnut/Cor a 8). To investigate Authors’ details whether an established Th2 immune response to LTPs 1 2 Paul-Ehrlich-Institut, Division of Allergology, Langen, Germany. University of facilitates a secondary immune response and cross-reactiv- Salzburg, Department of Molecular Biology, Salzburg, Austria. Thermo Fisher Scientific, Research and Development, Uppsala, Sweden. Paul-Ehrlich-Institut, ity to unrelated LTPs, CBA/J mice were immunized with Junior Research Group 1 Experimental Allergology, Langen, Germany. (1) Pru p 3 followed by Art v 3, (2) Art v 3 followed by Pru p 3, and (3) a mixture of Pru p 3 and Art v 3. Induc- Published: 17 March 2014 tion of Pru p 3 and Art v 3 specific IgE and IgG titers, and cross-reactivity between the two LTPs were determined by ELISA. CBA and C3H mice showed strong IgE-responses doi:10.1186/2045-7022-4-S2-O14 Cite this article as: Wangorsch et al.: LTP cross-reactivity - primary to Pru p 3, whereas BALB/c mice did not. Remarkably, a sensitization to mugwort pollen LTP Art v 3, facilitates subsequent species-specific immune response lacking any IgE- and sensitisation to peach LTP Pru p 3 in mice. Clinical and Translational Allergy IgG-cross-reactivity was observed for all tested LTPs 2014 4(Suppl 2):O14. except for Pru p 3 and Pru av 3. Although LTPs show high structural similarity, Prup3andArt v3displayed Paul-Ehrlich-Institut, Division of Allergology, Langen, Germany Full list of author information is available at the end of the article © 2014 Wangorsch et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

Journal

Clinical and Translational AllergySpringer Journals

Published: Mar 17, 2014

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