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Isolation, Purification, and Characterization of a Lectin from the Fungus Fusarium solani 4

Isolation, Purification, and Characterization of a Lectin from the Fungus Fusarium solani 4 Electrophoretically homogeneous lectin with a specific activity of 100.0 U/mg protein was isolated and purified from the fungal mycelium of Fusarium solani 4. The molecular weight of the native lectin molecule (38 kDa) was determined. It was established that it consists of two identical subunits, with the molecular mass determined via PAGE-Na electrophoresis in the presence of DDS-Na equal to 19 kDa. The physicochemical properties and carbohydrate specificity of the lectin, as well as its in vitro effect on mammalian cancer cells, were studied. The results opened new prospects for the further study of lectin and its potential applications as a medical agent. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Applied Biochemistry and Microbiology Springer Journals

Isolation, Purification, and Characterization of a Lectin from the Fungus Fusarium solani 4

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References (25)

Publisher
Springer Journals
Copyright
Copyright © Pleiades Publishing, Inc. 2021. ISSN 0003-6838, Applied Biochemistry and Microbiology, 2021, Vol. 57, No. 2, pp. 206–211. © Pleiades Publishing, Inc., 2021. Russian Text © The Author(s), 2021, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2021, Vol. 57, No. 2, pp. 145–151.
ISSN
0003-6838
eISSN
1608-3024
DOI
10.1134/S0003683821020095
Publisher site
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Abstract

Electrophoretically homogeneous lectin with a specific activity of 100.0 U/mg protein was isolated and purified from the fungal mycelium of Fusarium solani 4. The molecular weight of the native lectin molecule (38 kDa) was determined. It was established that it consists of two identical subunits, with the molecular mass determined via PAGE-Na electrophoresis in the presence of DDS-Na equal to 19 kDa. The physicochemical properties and carbohydrate specificity of the lectin, as well as its in vitro effect on mammalian cancer cells, were studied. The results opened new prospects for the further study of lectin and its potential applications as a medical agent.

Journal

Applied Biochemistry and Microbiology – Springer Journals

Published: Mar 12, 2021

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