Access the full text.
Sign up today, get DeepDyve free for 14 days.
R. Dawson, D. Elliott, W. Elliott (1986)
Data for Biochemical Research
Robert Holley, Robert Holley (1963)
Large-scale preparation of yeast "soluble" ribonucleic acid.Biochemical and biophysical research communications, 10
A. Crestfield, K. Smith, F. Allen (1955)
The preparation and characterization of ribonucleic acids from yeast.The Journal of biological chemistry, 216 1
D.N. Lazareva, E.K. i. Alekhin (1985)
Stimulyatory immuniteta
A simple method of production of total RNA from baker’s yeast was developed. Total RNA was isolated from yeast (Saccharomyces cerevisiae) biomass using lysis with sodium dodecyl sulfate at 100°C for 40–60 min and subsequent precipitation of the target product with 3 M NaCl. The preparation obtained was characterized in detail: yield of total RNA from 1 kg of pressed yeast, 9.25 g; optical density at 260 nm of 1 mg of RNA dissolved in 1 ml of water, 20.2 U; content of the acid-soluble fraction, 2.02%; and protein content, 1.8%. Total tRNA was isolated from total RNA by fractional precipitation with ethanol followed by gel filtration.
Applied Biochemistry and Microbiology – Springer Journals
Published: Feb 27, 2006
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.