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References (4)
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A. Molinero, D. Kingston, J. Reed (1989)
Biosynthesis of Antibiotics of the Virginiamycin Family, 6. Biosynthesis of Virginiamycin S1Journal of Natural Products, 52
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M. Giambattista, Gianni Chinali, Carlo Cocito (1989)
The molecular basis of the inhibitory activities of type A and type B synergimycins and related antibiotics on ribosomes.The Journal of antimicrobial chemotherapy, 24 4
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D. Kingston, M. Kolpak, J. Lefevre, I. Borup-Grochtmann (1983)
Biosynthesis of antibiotics of the virginiamycin family. 3. Biosynthesis of virginiamycin M1Journal of the American Chemical Society, 105
-
E. Vandamme (1984)
Biotechnology of industrial antibiotics
- Publisher
- Springer Journals
- Copyright
- Copyright © 2001 by MAIK “Nauka/Interperiodica”
- Subject
- Life Sciences; Medical Microbiology; Biochemistry, general; Microbiology
- ISSN
- 0003-6838
- eISSN
- 1608-3024
- DOI
- 10.1023/A:1010229117963
- Publisher site
- See Article on Publisher Site
Abstract
A method for chromatographic separation and quantitative determination of individual components of the antibiotic virginiamycin, produced by microbiological synthesis (Streptomyces virginiaestrain 147), is described. The components, M1–2and S1–5, were isolated from fermentation broth and identified by HPTLC and HPLC (the results obtained using the two methods correlate well with each other). The conditions of culturing of the producer and the composition of nutritive media were optimized. Using UV irradiation as a mutagenic factor, the producer was selected for increased level of synthesis of the antibiotic; this was achieved by inducing mutations that impart resistance to virginiamycin and meta-fluorophenylalanine, an analog of phenylalanine.
Journal
Applied Biochemistry and Microbiology – Springer Journals
Published: Oct 19, 2004