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International Standardisation of a Method for Detection of Human Pathogenic Viruses in Molluscan Shellfish

International Standardisation of a Method for Detection of Human Pathogenic Viruses in Molluscan... The viruses primarily associated with shellfish-borne illness are norovirus, causing gastroenteritis and hepatitis A virus (HAV). Recent years have seen a proliferation of publications on methods for detection of these viruses in shellfish using polymerase chain reaction (PCR). However, currently no standard harmonised procedures have been published. Standardisation is necessary before virus methods can be considered for adoption within a regulatory framework. A European standardisation working group is developing a two-part (quantitative and qualitative) standard method for virus detection in foodstuffs, including shellfish, which has the potential to be incorporated into EU legislation as a reference method. This article describes the development of the standard method and outlines the key methodology principles adopted, the controls and other quality assurance measures supporting the method and future necessary developments in the area. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Food and Environmental Virology Springer Journals

International Standardisation of a Method for Detection of Human Pathogenic Viruses in Molluscan Shellfish

Food and Environmental Virology , Volume 2 (3) – Jun 16, 2010

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Publisher
Springer Journals
Copyright
Copyright © 2010 by Her Majesty the Queen
Subject
Biomedicine; Chemistry/Food Science, general ; Food Science ; Virology
ISSN
1867-0334
eISSN
1867-0342
DOI
10.1007/s12560-010-9042-5
Publisher site
See Article on Publisher Site

Abstract

The viruses primarily associated with shellfish-borne illness are norovirus, causing gastroenteritis and hepatitis A virus (HAV). Recent years have seen a proliferation of publications on methods for detection of these viruses in shellfish using polymerase chain reaction (PCR). However, currently no standard harmonised procedures have been published. Standardisation is necessary before virus methods can be considered for adoption within a regulatory framework. A European standardisation working group is developing a two-part (quantitative and qualitative) standard method for virus detection in foodstuffs, including shellfish, which has the potential to be incorporated into EU legislation as a reference method. This article describes the development of the standard method and outlines the key methodology principles adopted, the controls and other quality assurance measures supporting the method and future necessary developments in the area.

Journal

Food and Environmental VirologySpringer Journals

Published: Jun 16, 2010

References