Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Increased Isoprene Production by the Recombinant Pantoea ananatis Strain due to the Balanced Amplification of Mevalonate Pathway Genes

Increased Isoprene Production by the Recombinant Pantoea ananatis Strain due to the Balanced... ISSN 0003-6838, Applied Biochemistry and Microbiology, 2019, Vol. 55, No. 9, pp. 850–860. © Pleiades Publishing, Inc., 2019. Russian Text © The Author(s), 2019, published in Biotekhnologiya, 2019, Vol. 35, No. 2, pp. 3–15. PRODUCERS, BIOLOGY, SELECTION, AND GENE ENGINEERING Increased Isoprene Production by the Recombinant Pantoea ananatis Strain due to the Balanced Amplif ication of Mevalonate Pathway Genes a a, b a J. I. Katashkina , E. D. Kazieva *, Y. Tajima , and S. V. Mashko Ajinomoto-Genetika Research Institute (AGRI) Closed Joint-Stock Company, Moscow, 117545 Russia Institute for Innovation, Ajinomoto Co., Inc., Kawasaki-ku, Kawasaki, 210-8681 Japan *e-mail: ekaterina_kazieva@agri.ru Received December 11, 2018; revised February 8, 2019; accepted March 15, 2019 Abstract—The uncoordinated expression of a target biosynthetic pathway often results in low growth and pro- ductivity for a producing strain due to the accumulation of toxic metabolic intermediates. In this study, we report an increase in isoprene accumulation by an engineered strain of Pantoea ananatis that possesses for- eign, artificial mevalonate-pathway genes. This was achieved due to the simultaneous, ϕ80-Int-dependent integration of a mixture of conditionally replicated plasmids carrying genes for the upper and lower meva- lonate pathways and mevalonate kinase, followed by selection according to the http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Applied Biochemistry and Microbiology Springer Journals

Increased Isoprene Production by the Recombinant Pantoea ananatis Strain due to the Balanced Amplification of Mevalonate Pathway Genes

Download PDF
11 pages

Loading next page...
 
/lp/springer-journals/increased-isoprene-production-by-the-recombinant-pantoea-ananatis-wG40c4VRHI
Publisher
Springer Journals
Copyright
Copyright © 2019 by Pleiades Publishing, Inc.
Subject
Life Sciences; Biochemistry, general; Microbiology; Medical Microbiology
ISSN
0003-6838
eISSN
1608-3024
DOI
10.1134/S0003683819090023
Publisher site
See Article on Publisher Site

Abstract

ISSN 0003-6838, Applied Biochemistry and Microbiology, 2019, Vol. 55, No. 9, pp. 850–860. © Pleiades Publishing, Inc., 2019. Russian Text © The Author(s), 2019, published in Biotekhnologiya, 2019, Vol. 35, No. 2, pp. 3–15. PRODUCERS, BIOLOGY, SELECTION, AND GENE ENGINEERING Increased Isoprene Production by the Recombinant Pantoea ananatis Strain due to the Balanced Amplif ication of Mevalonate Pathway Genes a a, b a J. I. Katashkina , E. D. Kazieva *, Y. Tajima , and S. V. Mashko Ajinomoto-Genetika Research Institute (AGRI) Closed Joint-Stock Company, Moscow, 117545 Russia Institute for Innovation, Ajinomoto Co., Inc., Kawasaki-ku, Kawasaki, 210-8681 Japan *e-mail: ekaterina_kazieva@agri.ru Received December 11, 2018; revised February 8, 2019; accepted March 15, 2019 Abstract—The uncoordinated expression of a target biosynthetic pathway often results in low growth and pro- ductivity for a producing strain due to the accumulation of toxic metabolic intermediates. In this study, we report an increase in isoprene accumulation by an engineered strain of Pantoea ananatis that possesses for- eign, artificial mevalonate-pathway genes. This was achieved due to the simultaneous, ϕ80-Int-dependent integration of a mixture of conditionally replicated plasmids carrying genes for the upper and lower meva- lonate pathways and mevalonate kinase, followed by selection according to the

Journal

Applied Biochemistry and Microbiology – Springer Journals

Published: Dec 5, 2019

References

  • Metabolic pathway engineering for production of 1,2-propanediol and 1-propanol by Corynebacterium glutamicum
    Siebert, D.; Wendisch, V.F.
  • Metabolic engineering of Escherichia coli for 1-butanol production
    Atsumi, S.; Cann, A.F.; Connor, M.R.
  • Metabolic engineering of Escherichia coli for the production of putrescine: a four carbon diamine
    Qian, Z.G.; Xia, X.X.; Lee, S.Y.
  • Heterologous expression of the mevalonic acid pathway in cyanobacteria enhances endogenous carbon partitioning to isoprene
    Bentley, F.K.; Zurbriggen, A.; Melis, A.
  • Transposon-based strategies for microbial functional genomics and proteomics
    Hayes, F.
  • Methods for generating precise deletions and insertions in the genome of wild-type Escherichia coli: application to open reading frame characterization
    Link, A.J.; Phillips, D.; Church, G.M.
  • A rapid and reliable strategy for chromosomal integration of gene(s) with multiple copies
    Gu, P.; Yang, F.; Su, T.
  • Conditional-replication, integration, excision, and retrieval plasmid-host systems for gene structure-function studies of bacteria
    Haldimann, A.; Wanner, B.L.
  • Convenient and reversible site-specific targeting of exogenous DNA into a bacterial chromosome by use of the FLP recombinase: the FLIRT system
    Huang, L.-C.; Wood, E.A.; Cox, M.M.
  • Use of site-specific recombination to regenerate selectable markers
    Cregg, J.M.; Madden, K.R.
  • LeeE., and Ow, D.W., Site-specific integration of DNA into wild-type and mutant lox sites placed in the plant genome
    Albert, H.; Dale, E.C.
  • The role of the loxP spacer region in PI site-specific recombination
    Hoess, R.H.; Wierzbicki, A.; Abremski, K.
  • Cre-lox-based system for multiple gene deletions and selectable-marker removal in Lactobacillus plantarum
    Lambert, J.M.; Bongers, R.S.; Kleerebezem, M.
  • Tuning the Expression level of a gene located on a bacterial chromosome
    Katashkina, Zh.I.; Skorokhodova, A.Yu.; Zimenkov, D.V.
  • Dual-In/Out strategy for genes integration into bacterial chromosome: a novel approach to step-by-step construction of plasmid-less marker-less recombinant E. coli strains with predesigned genome structure
    Minaeva, N.I.; Gak, E.R.; Zimenkov, D.V.
  • The complete genome sequence of Pantoea ananatis AJ13355, an organism with great biotechnological potential
    Hara, Y.; Kadotani, N.; Izui, H.
  • U. Use of the lambda Red-recombineering method for genetic engineering of Pantoea ananatis
    Katashkina, J.I.; Hara, Y.; Golubeva, L.I.
  • Combinatorial expression of bacterial whole mevalonate pathway for the production of beta-carotene in E. coli
    Yoon, S.H.; Lee, S.H.; Das, A.
  • The use of metabolic regulation to optimize gene expression in bacterial cells: a new direction in biotechnology of the 21st century
    Mashko, S.V.; Skorokhodova, A.Yu.; Zimenkov, D.V.
  • Engineering a mevalonate pathway in Escherichia coli for production of terpenoids
    Martin, V.J.; Pitera, D.J.; Withers, S.T.
  • Application of functional genomics to pathway optimization for increased isoprenoid production
    Kizer, L.; Pitera, D.J.; Pfleger, B.F.; Keasling, J.D.
  • Characterization of a feed-back resistant mevalonate kinase from the archaeon Methanosarcina mazei
    Primak, Y.A.; Du, M.; Miller, M.C.
  • Characterization of feedback-resistant mevalonate kinases from the methanogenic archaeons Methanosaeta concilii and Methanocella paludicola
    Kazieva, E.; Yamamoto, Y.; Tajima, Y.
  • Integrated analysis of isopentenyl pyrophosphate (IPP) toxicity in isoprenoid-producing Escherichia coli
    George, K.W.; Thompson, M.G.; Kim, J.
  • Isopentenyl diphosphate isomerase: a checkpoint to isoprenoid biosynthesis
    Berthelot, K.; Estevez, Y.; Deffieux, A.; Peruch, F.
  • Post-transcriptional control of gene expression: effectors of mRNA decay
    Arraiano, C.M.; Maquat, L.E.
  • Control of translation by mRNA secondary structure in Escherichia coli. A quantitative analysis of literature data
    de Smit, M.H.; van Dui, J.
  • Studies on lysogenesis. I. The mode of phage liberation by lysogenic Escherichia coli
    Bertani, G.
  • Transposon vectors containing non-antibiotic resistance selection markers for cloning and stable chromosomal insertion of foreign genes in gram-negative bacteria
    Herrero, M.; de Lorenzo, V.; Timmis, K.N.
  • Identification of Pantoea ananatis gene encoding membrane pyrroloquinoline quinone (PQQ)-dependent glucose dehydrogenase and pqqABCDEF operon essential for PQQ biosynthesis
    Andreeva, I.G.; Golubeva, L.I.; Kuvaeva, T.M.
  • E. coli chromosome regions that are more preferable for gene insertion, when the phage mu-driven system is used for integration
    Zimenkov, D.V.; Skorokhodova, A.Yu.; Katashkina, Zh. I.
  • Regulatory consequences of gene translocation in bacteria
    Block, D.H.S.; Hussein, R.; Liang, L.W.; Lim, H.N.
  • Purification and properties of formaldehyde dehydrogenase and formate dehydrogenase from Candida boidinii
    Schute, H.; Flossdorf, J.; Sahm, H.; Kula, M.R.
  • Applying asymptotic methods to synthetic biology: modelling the reaction kinetics of the mevalonate pathway
    Dalwadi, M.P.; Garavaglia, M.; Webb, J.P.
  • Use of T7 RNA polymerase to direct expression of cloned genes
    Studier, F.W.; Rosenberg, A.H.; Dunn, J.J.; Dubendorff, J.W.
  • A novel sequence element derived from bacteriophage T7 mRNA acts as an enhancer of translation of the lacZ gene in Escherichia coli
    Olins, P.O.; Rangwala, S.H.