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Increased Isoprene Production by the Recombinant Pantoea ananatis Strain due to the Balanced Amplification of Mevalonate Pathway Genes

Increased Isoprene Production by the Recombinant Pantoea ananatis Strain due to the Balanced... ISSN 0003-6838, Applied Biochemistry and Microbiology, 2019, Vol. 55, No. 9, pp. 850–860. © Pleiades Publishing, Inc., 2019. Russian Text © The Author(s), 2019, published in Biotekhnologiya, 2019, Vol. 35, No. 2, pp. 3–15. PRODUCERS, BIOLOGY, SELECTION, AND GENE ENGINEERING Increased Isoprene Production by the Recombinant Pantoea ananatis Strain due to the Balanced Amplif ication of Mevalonate Pathway Genes a a, b a J. I. Katashkina , E. D. Kazieva *, Y. Tajima , and S. V. Mashko Ajinomoto-Genetika Research Institute (AGRI) Closed Joint-Stock Company, Moscow, 117545 Russia Institute for Innovation, Ajinomoto Co., Inc., Kawasaki-ku, Kawasaki, 210-8681 Japan *e-mail: ekaterina_kazieva@agri.ru Received December 11, 2018; revised February 8, 2019; accepted March 15, 2019 Abstract—The uncoordinated expression of a target biosynthetic pathway often results in low growth and pro- ductivity for a producing strain due to the accumulation of toxic metabolic intermediates. In this study, we report an increase in isoprene accumulation by an engineered strain of Pantoea ananatis that possesses for- eign, artificial mevalonate-pathway genes. This was achieved due to the simultaneous, ϕ80-Int-dependent integration of a mixture of conditionally replicated plasmids carrying genes for the upper and lower meva- lonate pathways and mevalonate kinase, followed by selection according to the http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Applied Biochemistry and Microbiology Springer Journals

Increased Isoprene Production by the Recombinant Pantoea ananatis Strain due to the Balanced Amplification of Mevalonate Pathway Genes

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Publisher
Springer Journals
Copyright
Copyright © 2019 by Pleiades Publishing, Inc.
Subject
Life Sciences; Biochemistry, general; Microbiology; Medical Microbiology
ISSN
0003-6838
eISSN
1608-3024
DOI
10.1134/S0003683819090023
Publisher site
See Article on Publisher Site

Abstract

ISSN 0003-6838, Applied Biochemistry and Microbiology, 2019, Vol. 55, No. 9, pp. 850–860. © Pleiades Publishing, Inc., 2019. Russian Text © The Author(s), 2019, published in Biotekhnologiya, 2019, Vol. 35, No. 2, pp. 3–15. PRODUCERS, BIOLOGY, SELECTION, AND GENE ENGINEERING Increased Isoprene Production by the Recombinant Pantoea ananatis Strain due to the Balanced Amplif ication of Mevalonate Pathway Genes a a, b a J. I. Katashkina , E. D. Kazieva *, Y. Tajima , and S. V. Mashko Ajinomoto-Genetika Research Institute (AGRI) Closed Joint-Stock Company, Moscow, 117545 Russia Institute for Innovation, Ajinomoto Co., Inc., Kawasaki-ku, Kawasaki, 210-8681 Japan *e-mail: ekaterina_kazieva@agri.ru Received December 11, 2018; revised February 8, 2019; accepted March 15, 2019 Abstract—The uncoordinated expression of a target biosynthetic pathway often results in low growth and pro- ductivity for a producing strain due to the accumulation of toxic metabolic intermediates. In this study, we report an increase in isoprene accumulation by an engineered strain of Pantoea ananatis that possesses for- eign, artificial mevalonate-pathway genes. This was achieved due to the simultaneous, ϕ80-Int-dependent integration of a mixture of conditionally replicated plasmids carrying genes for the upper and lower meva- lonate pathways and mevalonate kinase, followed by selection according to the

Journal

Applied Biochemistry and MicrobiologySpringer Journals

Published: Dec 5, 2019

References