Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

In vivo assessment of the potential protective effect of Lactobacillus casei Shirota against aflatoxin B1

In vivo assessment of the potential protective effect of Lactobacillus casei Shirota against... Interest in the bacterial protective effect against mycotoxins has greatly increased. This effect is mostly strain-dependent and the mechanisms involved are still not well understood. The objectives of this work were (1) to assess the ability of Lactobacillus casei Shirota to bind aflatoxin B1 (AFB1) by fluorescent monoclonal antibody staining, (2) to evaluate the AFB1-bacteria interaction by atomic force microscopy, and (3) to determine its effect on intestinal absorption through detection of AFB1-Lys adducts used as biological markers to a 3-week aflatoxin exposure in a murine model. The micrographs obtained in this work showed for the first time a clear visual image of the ability of Lactobacillus casei Shirota to bind AFB1 into the bacterial cell envelope. The images also revealed that aflatoxin binding produces structural changes that modify the bacterial cell surface. AFB1-Lys adducts quantified from blood samples were found to be present at significantly lower levels in animals receiving AFB1 plus bacteria than in those receiving only AFB1. This suggests that the presence of Lactobacillus casei Shirota can decrease aflatoxin absorption at the intestinal level even after a long period of toxin exposure, which consequently circumvents its toxic effects. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Dairy Science & Technology Springer Journals

In vivo assessment of the potential protective effect of Lactobacillus casei Shirota against aflatoxin B1

Loading next page...
 
/lp/springer-journals/in-vivo-assessment-of-the-potential-protective-effect-of-lactobacillus-3KJ4xmnpec
Publisher
Springer Journals
Copyright
Copyright © 2010 by Springer S+B Media B.V.
Subject
Chemistry; Food Science; Agriculture; Microbiology
ISSN
1958-5586
eISSN
1958-5594
DOI
10.1051/dst/2010030
Publisher site
See Article on Publisher Site

Abstract

Interest in the bacterial protective effect against mycotoxins has greatly increased. This effect is mostly strain-dependent and the mechanisms involved are still not well understood. The objectives of this work were (1) to assess the ability of Lactobacillus casei Shirota to bind aflatoxin B1 (AFB1) by fluorescent monoclonal antibody staining, (2) to evaluate the AFB1-bacteria interaction by atomic force microscopy, and (3) to determine its effect on intestinal absorption through detection of AFB1-Lys adducts used as biological markers to a 3-week aflatoxin exposure in a murine model. The micrographs obtained in this work showed for the first time a clear visual image of the ability of Lactobacillus casei Shirota to bind AFB1 into the bacterial cell envelope. The images also revealed that aflatoxin binding produces structural changes that modify the bacterial cell surface. AFB1-Lys adducts quantified from blood samples were found to be present at significantly lower levels in animals receiving AFB1 plus bacteria than in those receiving only AFB1. This suggests that the presence of Lactobacillus casei Shirota can decrease aflatoxin absorption at the intestinal level even after a long period of toxin exposure, which consequently circumvents its toxic effects.

Journal

Dairy Science & TechnologySpringer Journals

Published: Jul 4, 2011

References