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Improved Toxicological Profile and Tumoricidal Activity of Doxorubicin in Combination with Mycosynthesized Pt Nanoparticles in a Sarcoma-180 Transplantable Tumor Model

Improved Toxicological Profile and Tumoricidal Activity of Doxorubicin in Combination with... Doxorubicin (Dox) is a potent and widely used chemotherapeutic drug. However, it has poor tissue penetration contributing to a low therapeutic index which severely limits the effectiveness of this anticancer drug. Nanoparticle-based delivery of Dox is a major approach to enhance its tissue penetration leading to enhanced efficacy at lower doses. In this study, platinum nanoparticles (PtNP) synthesized from the phytopathogenic fungus Curvularia affinis (CaPtNP), was used in combination with Dox in vitro in sarcoma-180 cells (S-180) and in vivo in S-180 transplantable tumor-bearing Swiss albino mice. IC 10, 20 and 30 concentrations of Dox and CaPtNP alone and in various combinations were used to treat S180 cells in vitro. Cell count and cell cycle study depicted more effective results in presence of increasing CaPtNP concentration with Dox. Moreover, fluorescence images and flowcytometric measurements showed increased Dox fluorescence in the combination treatment groups signifying enhanced cellular uptake of Dox in presence of CaPtNP. A similar trend ensued in vivo in tumor-bearing mice with a significantly greater reduction of tumor burden in the combination group than other groups. The enhanced anticancer effect was accompanied by an improved hepato-, myelo- and cardiotoxicity profile in the Dox + CaPtNP group. This study provides an enticing platform to develop Dox carrier which would reduce toxicity without compromising its anticancer property. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Proceedings of the Zoological Society Springer Journals

Improved Toxicological Profile and Tumoricidal Activity of Doxorubicin in Combination with Mycosynthesized Pt Nanoparticles in a Sarcoma-180 Transplantable Tumor Model

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Publisher
Springer Journals
Copyright
Copyright © Zoological Society, Kolkata, India 2022. Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.
ISSN
0373-5893
eISSN
0974-6919
DOI
10.1007/s12595-022-00454-1
Publisher site
See Article on Publisher Site

Abstract

Doxorubicin (Dox) is a potent and widely used chemotherapeutic drug. However, it has poor tissue penetration contributing to a low therapeutic index which severely limits the effectiveness of this anticancer drug. Nanoparticle-based delivery of Dox is a major approach to enhance its tissue penetration leading to enhanced efficacy at lower doses. In this study, platinum nanoparticles (PtNP) synthesized from the phytopathogenic fungus Curvularia affinis (CaPtNP), was used in combination with Dox in vitro in sarcoma-180 cells (S-180) and in vivo in S-180 transplantable tumor-bearing Swiss albino mice. IC 10, 20 and 30 concentrations of Dox and CaPtNP alone and in various combinations were used to treat S180 cells in vitro. Cell count and cell cycle study depicted more effective results in presence of increasing CaPtNP concentration with Dox. Moreover, fluorescence images and flowcytometric measurements showed increased Dox fluorescence in the combination treatment groups signifying enhanced cellular uptake of Dox in presence of CaPtNP. A similar trend ensued in vivo in tumor-bearing mice with a significantly greater reduction of tumor burden in the combination group than other groups. The enhanced anticancer effect was accompanied by an improved hepato-, myelo- and cardiotoxicity profile in the Dox + CaPtNP group. This study provides an enticing platform to develop Dox carrier which would reduce toxicity without compromising its anticancer property.

Journal

Proceedings of the Zoological SocietySpringer Journals

Published: Dec 1, 2022

Keywords: Doxorubicin (Dox); Nanoparticle; Sarcoma180; Anticancer effect; Toxicity

References