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Immunochromatographic Test Systems for Detection of Microcystin-LR in Seafood

Immunochromatographic Test Systems for Detection of Microcystin-LR in Seafood For the rapid control of the highly toxic phycotoxin microcystin-LR in seafood, immunochromatographic test systems have been developed and compared using two types of gold nanoparticles as markers, namely, spherical particles and nanoflowers. The test systems are based on an indirect competitive assay, in which the detectable immune complexes include native microcystin-LR-specific antibodies and nanoparticle-labeled anti-species antibodies. The developed test systems allow for the detection of microcystin-LR within 18 min with instrumental detection limits of 0.2 and 0.1 ng/mL when using spherical nanoparticles and gold nanoflowers, respectively. The visual detection limit is 1 ng/mL for both formats. The parameters of nanoparticles, which determine the analytical characteristics of the corresponding test systems are characterized. The applicability of the developed test systems for the control of contamination of fish and seafood (shrimp, octopus, squid, and mussels) combining rapid stages of sample preparation and immunochromatography has been shown. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Applied Biochemistry and Microbiology Springer Journals

Immunochromatographic Test Systems for Detection of Microcystin-LR in Seafood

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Publisher
Springer Journals
Copyright
Copyright © Pleiades Publishing, Inc. 2021. ISSN 0003-6838, Applied Biochemistry and Microbiology, 2021, Vol. 57, No. 3, pp. 403–409. © Pleiades Publishing, Inc., 2021. Russian Text © The Author(s), 2021, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2021, Vol. 57, No. 3, pp. 303–310.
ISSN
0003-6838
eISSN
1608-3024
DOI
10.1134/s0003683821030170
Publisher site
See Article on Publisher Site

Abstract

For the rapid control of the highly toxic phycotoxin microcystin-LR in seafood, immunochromatographic test systems have been developed and compared using two types of gold nanoparticles as markers, namely, spherical particles and nanoflowers. The test systems are based on an indirect competitive assay, in which the detectable immune complexes include native microcystin-LR-specific antibodies and nanoparticle-labeled anti-species antibodies. The developed test systems allow for the detection of microcystin-LR within 18 min with instrumental detection limits of 0.2 and 0.1 ng/mL when using spherical nanoparticles and gold nanoflowers, respectively. The visual detection limit is 1 ng/mL for both formats. The parameters of nanoparticles, which determine the analytical characteristics of the corresponding test systems are characterized. The applicability of the developed test systems for the control of contamination of fish and seafood (shrimp, octopus, squid, and mussels) combining rapid stages of sample preparation and immunochromatography has been shown.

Journal

Applied Biochemistry and Microbiology – Springer Journals

Published: Jun 3, 2021

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