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Identification of the Stress Hli Protein in the Pigment–Protein Complexes of Arthrospira platensis

Identification of the Stress Hli Protein in the Pigment–Protein Complexes of Arthrospira platensis Hli proteins of the multicellular cyanobacterium Arthrospira platensis have been studied in this work. According to the NCBI database, three Hli genes found in the A. platensis genome encode proteins 47, 64, and 69 amino acid (aa). A. platensis cells were incubated under light stress (500 µmol photons/m2 s, 1 h). The association of Hli proteins with pigment–protein complexes of thylakoid membranes was then studied by two-dimensional electrophoresis and subsequent mass spectrometry. Only Hli 47 aa was detected in the composition of pigment–protein complexes by matrix-assisted laser desorption/ionization–time-of-flight (MALDI-TOF) mass spectrometry. The identified Hli47 protein was shown to be associated with photosystem II and a homolog of the HliC protein of Synechocystis sp. Bioinformatic analysis has shown that the amino acid sequence of the identified protein shows a higher homology with proteins of multicellular cyanobacteria and a lower of homology with the amino acid sequence of Hli proteins of unicellular cyanobacteria. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Applied Biochemistry and Microbiology Springer Journals

Identification of the Stress Hli Protein in the Pigment–Protein Complexes of Arthrospira platensis

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References (27)

Publisher
Springer Journals
Copyright
Copyright © Pleiades Publishing, Inc. 2021. ISSN 0003-6838, Applied Biochemistry and Microbiology, 2021, Vol. 57, No. 6, pp. 706–711. © Pleiades Publishing, Inc., 2021. Russian Text © The Author(s), 2021, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2021, Vol. 57, No. 6, pp. 549–555.
ISSN
0003-6838
eISSN
1608-3024
DOI
10.1134/s0003683821060119
Publisher site
See Article on Publisher Site

Abstract

Hli proteins of the multicellular cyanobacterium Arthrospira platensis have been studied in this work. According to the NCBI database, three Hli genes found in the A. platensis genome encode proteins 47, 64, and 69 amino acid (aa). A. platensis cells were incubated under light stress (500 µmol photons/m2 s, 1 h). The association of Hli proteins with pigment–protein complexes of thylakoid membranes was then studied by two-dimensional electrophoresis and subsequent mass spectrometry. Only Hli 47 aa was detected in the composition of pigment–protein complexes by matrix-assisted laser desorption/ionization–time-of-flight (MALDI-TOF) mass spectrometry. The identified Hli47 protein was shown to be associated with photosystem II and a homolog of the HliC protein of Synechocystis sp. Bioinformatic analysis has shown that the amino acid sequence of the identified protein shows a higher homology with proteins of multicellular cyanobacteria and a lower of homology with the amino acid sequence of Hli proteins of unicellular cyanobacteria.

Journal

Applied Biochemistry and MicrobiologySpringer Journals

Published: Nov 1, 2021

Keywords: photosystem II; light stress; light-induced stress proteins; Hli proteins (high light inducible proteins)

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