Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Identification of resistance in tomato against root knot nematode (Meloidogyne incognita) and comparison of molecular markers for Mi gene

Identification of resistance in tomato against root knot nematode (Meloidogyne incognita) and... Tomato production is limited by many biotic stresses of which root knot nematode (RKN, Meloidogyne incognita) is a major pest. The present study aimed to identify resistance sources in controlled conditions and compare molecular markers for efficient and rapid screening of M. incognita resistance. Among the ten genotypes evaluated, HAT-310 and HAT-311 were found immune to M. incognita infestation. Further, six crosses with these two resistant sources, (HAT-311 x Swarna Lalima, HAT-296 x HAT-311, EC-596747 x HAT-311, Swarna Lalima x HAT-310, EC-596743 x HAT-310 and Swarna Lalima x HAT-311), exhibited immune responses against M. incognita. Four molecular markers viz. JB-1, REX-1, PMi12 and Mi23 were employed in eighteen germplasm to characterise resistance and susceptibility of the genotypes against infestation by M. incognita. JB-1 yielded 420 bp in all the genotypes after digestion and hence could not be used to differentiate between nematode resistance and susceptibility. Marker PMi12 yielded additional DNA fragments in addition to the expected bands and did not give consistent results. REX-1 and Mi23 markers successfully differentiated between nematode resistant and susceptible genotypes. Moreover, Mi23 separated the homozygous and heterozygous resistance sources since the restriction enzyme analysis was not needed. The resistant genotypes identified from the present study may be used further in nematode resistance breeding programmes of tomato and the Mi23 marker can be used for rapid screening of the germplasm. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Australasian Plant Pathology Springer Journals

Identification of resistance in tomato against root knot nematode (Meloidogyne incognita) and comparison of molecular markers for Mi gene

Loading next page...
 
/lp/springer-journals/identification-of-resistance-in-tomato-against-root-knot-nematode-O8KzDziNwF

References (32)

Publisher
Springer Journals
Copyright
Copyright © 2018 by Australasian Plant Pathology Society Inc.
Subject
Life Sciences; Plant Pathology; Plant Sciences; Agriculture; Entomology; Ecology
ISSN
0815-3191
eISSN
1448-6032
DOI
10.1007/s13313-018-0602-8
Publisher site
See Article on Publisher Site

Abstract

Tomato production is limited by many biotic stresses of which root knot nematode (RKN, Meloidogyne incognita) is a major pest. The present study aimed to identify resistance sources in controlled conditions and compare molecular markers for efficient and rapid screening of M. incognita resistance. Among the ten genotypes evaluated, HAT-310 and HAT-311 were found immune to M. incognita infestation. Further, six crosses with these two resistant sources, (HAT-311 x Swarna Lalima, HAT-296 x HAT-311, EC-596747 x HAT-311, Swarna Lalima x HAT-310, EC-596743 x HAT-310 and Swarna Lalima x HAT-311), exhibited immune responses against M. incognita. Four molecular markers viz. JB-1, REX-1, PMi12 and Mi23 were employed in eighteen germplasm to characterise resistance and susceptibility of the genotypes against infestation by M. incognita. JB-1 yielded 420 bp in all the genotypes after digestion and hence could not be used to differentiate between nematode resistance and susceptibility. Marker PMi12 yielded additional DNA fragments in addition to the expected bands and did not give consistent results. REX-1 and Mi23 markers successfully differentiated between nematode resistant and susceptible genotypes. Moreover, Mi23 separated the homozygous and heterozygous resistance sources since the restriction enzyme analysis was not needed. The resistant genotypes identified from the present study may be used further in nematode resistance breeding programmes of tomato and the Mi23 marker can be used for rapid screening of the germplasm.

Journal

Australasian Plant PathologySpringer Journals

Published: Nov 17, 2018

There are no references for this article.