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Identification and optimization of ISSR primers for analysis of genetic variability among different landrace collections of Indian sponge gourd (Luffa cylindrica L.)

Identification and optimization of ISSR primers for analysis of genetic variability among... Luffa cylindrica L. or sponge gourd is an economically important horticultural crop from Indian origin and belongs to family Cucurbitaceae. It has extremely variable characteristics like fruit shape, size, quality etc. Though, the genetic variety and population structure in Luffa species is not well implicited neither previously reported accurately. Thus, present work aims to optimize methodology for identification of genetic diversity and population structure among its different varieties and landrace assortments using PCR based (ISSR) markers. The study will help us in designing investigation for large scale characterization of genetic variability across different L. cylindrica. germplasm plus gene pool which could benefit breeding programs. In the present work ISSR markers were used in genetic profiling of 58 sponge gourd accessions using five selected ISSR primers. The landrace collection which were giving maximum amplification were then phylogenetically analyzed to identify their closest relationship with other varieties of Sponge gourd. The three landrace (Luffa-886, Luffa-889 and Luffa-890) germplasm were carefully chosen based on their polymorphic results and phylogenetic relationship were established. This study also provides information for Luffa genotypes, as well as the polymorphic markers identified could be available for the study of landmarks in linkages, evolutionary history and marker-assisted selection in Luffa species. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Indian Journal of Plant Physiology Springer Journals

Identification and optimization of ISSR primers for analysis of genetic variability among different landrace collections of Indian sponge gourd (Luffa cylindrica L.)

Indian Journal of Plant Physiology , Volume 24 (3) – Sep 6, 2019

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References (27)

Publisher
Springer Journals
Copyright
Copyright © 2019 by Indian Society for Plant Physiology
Subject
Life Sciences; Plant Sciences; Plant Physiology; Plant Ecology; Plant Biochemistry; Cell Biology; Plant Genetics and Genomics
ISSN
0019-5502
eISSN
2662-2548
DOI
10.1007/s40502-019-00454-y
Publisher site
See Article on Publisher Site

Abstract

Luffa cylindrica L. or sponge gourd is an economically important horticultural crop from Indian origin and belongs to family Cucurbitaceae. It has extremely variable characteristics like fruit shape, size, quality etc. Though, the genetic variety and population structure in Luffa species is not well implicited neither previously reported accurately. Thus, present work aims to optimize methodology for identification of genetic diversity and population structure among its different varieties and landrace assortments using PCR based (ISSR) markers. The study will help us in designing investigation for large scale characterization of genetic variability across different L. cylindrica. germplasm plus gene pool which could benefit breeding programs. In the present work ISSR markers were used in genetic profiling of 58 sponge gourd accessions using five selected ISSR primers. The landrace collection which were giving maximum amplification were then phylogenetically analyzed to identify their closest relationship with other varieties of Sponge gourd. The three landrace (Luffa-886, Luffa-889 and Luffa-890) germplasm were carefully chosen based on their polymorphic results and phylogenetic relationship were established. This study also provides information for Luffa genotypes, as well as the polymorphic markers identified could be available for the study of landmarks in linkages, evolutionary history and marker-assisted selection in Luffa species.

Journal

Indian Journal of Plant PhysiologySpringer Journals

Published: Sep 6, 2019

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