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Identification and Functional Characterization of the DcF3’H Promoter from Dracaena cambodiana

Identification and Functional Characterization of the DcF3’H Promoter from Dracaena cambodiana Dragon’s blood is a traditional medicine that is produced mainly from Dracaenas plants and is widely used in the world. The main chemical compounds in dragon’s blood are flavonoids. Flavonoid 3′-hydroxylase (F3’H) is an enzyme that synthesizes 3′-hydroxylated flavonoids. DcF3’H plays a vital role in determining the flavonoid content during the production of dragon′s blood; however, the regulation of DcF3’H expression in Dracaenas plants is poorly understood. In this study, a 759-bp promoter region of DcF3’H was isolated from Dracaena cambodiana. The promoter sequence contained cis-acting elements related to various environmental stresses and hormones responses. Dual-luciferase assays showed that the DcF3’H promoter was up-regulated by 6-benzyl aminopurine (6-BA) and down-regulated by methyl jasmonate (MeJA), and these results were consistent with transcription levels of DcF3’H in stems that were verified by quantitative real-time PCR (qPCR). DcHLH1, a bHLH transcription factor identified in yeast one-hybrid screening, was capable of both binding to the promoter and activating the transcription of DcF3’H by ~10-fold. The results provide new insights into the regulatory mechanisms of DcF3’H in Dracaena cambodiana. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Tropical Plant Biology Springer Journals

Identification and Functional Characterization of the DcF3’H Promoter from Dracaena cambodiana

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References (54)

Publisher
Springer Journals
Copyright
Copyright © 2018 by Springer Science+Business Media, LLC, part of Springer Nature
Subject
Life Sciences; Plant Sciences; Plant Genetics and Genomics; Plant Breeding/Biotechnology; Plant Ecology; Transgenics
ISSN
1935-9756
eISSN
1935-9764
DOI
10.1007/s12042-018-9211-5
Publisher site
See Article on Publisher Site

Abstract

Dragon’s blood is a traditional medicine that is produced mainly from Dracaenas plants and is widely used in the world. The main chemical compounds in dragon’s blood are flavonoids. Flavonoid 3′-hydroxylase (F3’H) is an enzyme that synthesizes 3′-hydroxylated flavonoids. DcF3’H plays a vital role in determining the flavonoid content during the production of dragon′s blood; however, the regulation of DcF3’H expression in Dracaenas plants is poorly understood. In this study, a 759-bp promoter region of DcF3’H was isolated from Dracaena cambodiana. The promoter sequence contained cis-acting elements related to various environmental stresses and hormones responses. Dual-luciferase assays showed that the DcF3’H promoter was up-regulated by 6-benzyl aminopurine (6-BA) and down-regulated by methyl jasmonate (MeJA), and these results were consistent with transcription levels of DcF3’H in stems that were verified by quantitative real-time PCR (qPCR). DcHLH1, a bHLH transcription factor identified in yeast one-hybrid screening, was capable of both binding to the promoter and activating the transcription of DcF3’H by ~10-fold. The results provide new insights into the regulatory mechanisms of DcF3’H in Dracaena cambodiana.

Journal

Tropical Plant Biology – Springer Journals

Published: Oct 16, 2018

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